Ramaswamy Yogambha, Wu Chengtie, Zhou Hong, Zreiqat Hala
Biomaterials and Tissue Engineering Research Unit, Biomedical Engineering, School of AMME, The University of Sydney, Sydney, NSW, Australia.
Acta Biomater. 2008 Sep;4(5):1487-97. doi: 10.1016/j.actbio.2008.04.014. Epub 2008 May 3.
Calcium silicate (CaSiO(3)) ceramics have received considerable attention in recent years due to their excellent bioactivity and degradability. However, their poor chemical stability limits their biological applications. Hardystonite (Ca(2)ZnSi(2)O(7)) ceramics are Ca-Si-based materials developed by incorporating zinc into the Ca-Si system to improve their chemical stability. However, the biological responses of Ca(2)ZnSi(2)O(7) to bone cells are unknown. The objective of this study is to investigate and compare the in vitro responses of human osteoblast-like cells (HOBs) and osteoclasts when cultured on Ca(2)ZnSi(2)O(7) and CaSiO(3) ceramic disks. The ability of Ca(2)ZnSi(2)O(7) ceramics to support HOB attachment, cytoskeleton organization, proliferation and differentiation was assessed by scanning electron microscopy, confocal microscopy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, alkaline phosphatase activity and quantitative real-time polymerase chain reaction. Our results show that Ca(2)ZnSi(2)O(7) supported HOB attachment with a well-organized cytoskeleton structure, and significantly increased cellular proliferation and differentiation compared to CaSiO(3). In addition, Ca(2)ZnSi(2)O(7) showed increased expression levels of osteoblast-related mRNAs (alkaline phosphatase, collagen type I, osteocalcin, receptor activator of NF(kappa)B ligand and osteoprotegerin) compared to CaSiO(3). Ca(2)ZnSi(2)O(7) ceramic supported the formation of mature and functional osteoclasts and formed resorption imprints. On CaSiO(3) ceramics, the cells failed to differentiate from the monocytes into osteoclasts. Taken together, these results indicate that Hardystonite ceramics are conducive to both types of bone cells, osteoblast-like cells and osteoclasts, suggesting their potential use for skeletal tissue regeneration and as coatings onto currently available orthopedic and dental implants.
近年来，硅酸钙（CaSiO₃）陶瓷因其优异的生物活性和可降解性而受到广泛关注。然而，其较差的化学稳定性限制了它们的生物学应用。钙硅锌矿（Ca₂ZnSi₂O₇）陶瓷是通过将锌引入Ca-Si体系而开发的Ca-Si基材料，以提高其化学稳定性。然而，Ca₂ZnSi₂O₇对骨细胞的生物学反应尚不清楚。本研究的目的是研究和比较人成骨样细胞（HOB）和破骨细胞在Ca₂ZnSi₂O₇和CaSiO₃陶瓷盘上培养时的体外反应。通过扫描电子显微镜、共聚焦显微镜、3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法、碱性磷酸酶活性和定量实时聚合酶链反应评估Ca₂ZnSi₂O₇陶瓷支持HOB附着、细胞骨架组织、增殖和分化的能力。我们的结果表明，Ca₂ZnSi₂O₇支持HOB附着并具有组织良好的细胞骨架结构，与CaSiO₃相比，显著增加了细胞增殖和分化。此外，与CaSiO₃相比，Ca₂ZnSi₂O₇显示成骨细胞相关mRNA（碱性磷酸酶、I型胶原蛋白、骨钙素、核因子κB受体活化因子配体和骨保护素）的表达水平增加。Ca₂ZnSi₂O₇陶瓷支持成熟且功能正常的破骨细胞形成并形成吸收印记。在CaSiO₃陶瓷上，细胞未能从单核细胞分化为破骨细胞。综上所述，这些结果表明钙硅锌矿陶瓷有利于成骨样细胞和破骨细胞这两种类型的骨细胞，表明它们在骨骼组织再生以及作为目前可用的骨科和牙科植入物涂层方面具有潜在用途。
