Developmental Biology Laboratory, Animal Biology Department, School of Biology, College of Science, University of Tehran, Tehran 1417935840, Iran.
Department of Biotechnology, College of Science, University of Tehran, Tehran 1417935840, Iran.
Int J Mol Sci. 2021 Sep 25;22(19):10332. doi: 10.3390/ijms221910332.
Steroid-associated osteonecrosis (SAON) is a chronic disease that leads to the destruction and collapse of bone near the joint that is subjected to weight bearing, ultimately resulting in a loss of hip and knee function. Zn ions, as an essential trace element, have functional roles in improving the immunophysiological cellular environment, accelerating bone regeneration, and inhibiting biofilm formation. In this study, we reconstruct SAON lesions with a three-dimensional (3D)-a printed composite made of poly (epsilon-caprolactone) (PCL) and nanoparticulate Willemite (npW). Rabbit bone marrow stem cells were used to evaluate the cytocompatibility and osteogenic differentiation capability of the PCL/npW composite scaffolds. The 2-month bone regeneration was assessed by a Micro-computed tomography (micro-CT) scan and the expression of bone regeneration proteins by Western blot. Compared with the neat PCL group, PCL/npW scaffolds exhibited significantly increased cytocompatibility and osteogenic activity. This finding reveals a new concept for the design of a 3D-printed PCL/npW composite-based bone substitute for the early treatment of osteonecrosis defects.
类固醇相关骨坏死（SAON）是一种慢性疾病，会导致承重关节附近的骨破坏和塌陷，最终导致髋关节和膝关节功能丧失。Zn 离子作为一种必需的微量元素，具有改善免疫生理细胞环境、加速骨再生和抑制生物膜形成的功能作用。在这项研究中，我们使用三维（3D）打印的聚己内酯（PCL）和纳米颗粒硅灰石（npW）复合材料重建了 SAON 病变。兔骨髓干细胞用于评估 PCL/npW 复合支架的细胞相容性和成骨分化能力。通过微计算机断层扫描（micro-CT）扫描评估了 2 个月的骨再生情况，并通过 Western blot 评估了骨再生蛋白的表达。与纯 PCL 组相比，PCL/npW 支架表现出显著提高的细胞相容性和成骨活性。这一发现为设计基于 3D 打印的 PCL/npW 复合材料的骨替代物提供了一个新概念，用于早期治疗骨坏死缺陷。
