Vestergaard Else Marie, Nexo Ebba, Wendt Anke, Guthmann Florian
Department of Clinical Biochemistry, Aarhus University Hospital Skejby, Aarhus, Denmark.
Early Hum Dev. 2008 Oct;84(10):631-5. doi: 10.1016/j.earlhumdev.2008.04.001. Epub 2008 May 23.
We measured concentrations of the gastrointestinal protective peptides Trefoil factors in human milk. By the use of in-house ELISA we detected high amounts of TFF3, less TFF1 and virtually no TFF2 in human breast milk obtained from 46 mothers with infants born extremely preterm (24-27 wk gestation), preterm (28-37 wk gestation), and full term (38-42 wk gestation). Samples were collected during the first, second, third to fourth weeks and more than 4 wks postpartum. Median (range) TFF1 [TFF3] concentrations in human milk were 320 (30-34000) [1500 (150-27,000)] pmol/L in wk 1, 120 (30-720) [310 (50-7100)] pmol/L in wk 2, 70 (20-670) [120 (20-650)] pmol/L in wks 3 to 4, and 60 (30-2500) [80 (20-540)] pmol/L in >4 wks after delivery. The lowest concentrations of TFF1 and TFF3 were found later than 2 wks after birth. In conclusion, TFF was present in term and preterm human milk with rapidly declining concentrations during the first weeks post partum. The clinical significance of TFF present in human milk remains to be explored, both regarding development of the fetal gut and protection against necrotizing enterocolitis.
我们测量了人乳中胃肠道保护肽三叶因子的浓度。通过使用内部酶联免疫吸附测定法（ELISA），我们在从46名母亲采集的人乳中检测到了大量的三叶因子3（TFF3）、较少的三叶因子1（TFF1），而三叶因子2（TFF2）几乎未检测到。这些母亲的婴儿分别为极早产儿（妊娠24 - 27周）、早产儿（妊娠28 - 37周）和足月儿（妊娠38 - 42周）。样本在产后第一周、第二周、第三至第四周以及产后4周以上采集。人乳中TFF1 [TFF3]浓度的中位数（范围）在第1周为320（30 - 34000）[1500（150 - 27000）] pmol/L，第2周为120（30 - 720）[310（50 - 7100）] pmol/L，第3至4周为70（20 - 670）[120（20 - 650）] pmol/L，产后4周以上为60（30 - 2500）[80（20 - 540）] pmol/L。TFF1和TFF3的最低浓度在出生后2周后出现。总之，足月和早产人乳中均存在三叶因子，且在产后第一周内其浓度迅速下降。人乳中三叶因子的临床意义，无论是对胎儿肠道发育还是预防坏死性小肠结肠炎，仍有待探索。
