大鼠再生肝中前列腺素E2受体的下调及其生理意义。

Down-regulation of prostaglandin E2 receptors in regenerating rat liver and its physiological significance.

作者信息

Hashimoto N, Watanabe T, Ikeda Y, Toda G, Yamada H, Yoshikawa Y, Mitsui H, Kurokawa K

机构信息

First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Apr 15;176(1):226-32. doi: 10.1016/0006-291x(91)90913-r.

DOI:10.1016/0006-291x(91)90913-r

PMID:1850259

Abstract

The properties of prostaglandin (PG) E2 receptors in regenerating liver were studied using rat hepatocytes in primary culture. The control cells possessed stereo-specific PGE2 receptors with Bmax and Kd values, at 4 degrees C, of 526 fmol/mg protein and 6.5 nM respectively. In cells from regenerating liver after 70% hepatectomy, Bmax was reduced to 42-43% that of the controls; Kd did not change. Administration of indomethacin before surgery prevented Bmax reduction. These results indicate that PGE2, produced during the regeneration process, evoked cellular events and regulated the density of its receptors.

摘要

利用原代培养的大鼠肝细胞研究了再生肝中前列腺素（PG）E2受体的特性。对照细胞具有立体特异性PGE2受体，在4℃时，Bmax和Kd值分别为526 fmol/mg蛋白和6.5 nM。在70%肝切除术后再生肝的细胞中，Bmax降至对照细胞的42 - 43%；Kd未改变。术前给予吲哚美辛可防止Bmax降低。这些结果表明，再生过程中产生的PGE2引发了细胞事件并调节了其受体的密度。