Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Dig Dis Sci. 1995 Jun;40(6):1213-20. doi: 10.1007/BF02065526.
To determine whether an antibody against hepatic sinusoidal endothelial cells was present in sera of patients with autoimmune hepatitis (AIH) type 1, we measured the serum IgG bound to the glutaraldehyde-fixed cultured rat sinusoidal endothelial cells by enzyme-linked immunosorbent assay. IgG bound to the cells was detected significantly more in patients with autoimmune hepatitis type 1 (97.1%) than in those with primary biliary cirrhosis (13.0%), chronic hepatitis C (5.9%) or B (7.9%), or healthy controls (0%). IgG-F(ab')2 fragments from autoimmune hepatitis patients also bound to the cells, and this binding was observed after absorption of the fragments with rat hepatoma cells, but not after absorption with bovine carotid endothelial cells. Culture of sinusoidal endothelial cells in the presence of IgG from AIH patients significantly reduced the number of viable attached cells. In conclusion, anti-sinusoidal endothelial cell antibody occurred in the sera from patients with autoimmune hepatitis type 1.
为了确定1型自身免疫性肝炎（AIH）患者血清中是否存在抗肝窦内皮细胞抗体，我们采用酶联免疫吸附测定法检测了与戊二醛固定的培养大鼠肝窦内皮细胞结合的血清IgG。1型自身免疫性肝炎患者中与细胞结合的IgG检测阳性率（97.1%）显著高于原发性胆汁性肝硬化患者（13.0%）、丙型肝炎（5.9%）或乙型肝炎患者（7.9%）以及健康对照者（0%）。来自自身免疫性肝炎患者的IgG-F(ab')2片段也与细胞结合，且在用大鼠肝癌细胞吸收片段后仍可观察到这种结合，但在用牛颈动脉内皮细胞吸收后则未观察到。在AIH患者的IgG存在下培养肝窦内皮细胞，显著减少了存活贴壁细胞的数量。总之，1型自身免疫性肝炎患者血清中存在抗肝窦内皮细胞抗体。
