Liu Xiaoling, Liu He, Chen Jinhuan, Du Guocheng, Chen Jian
School of Biotechnology, Southern Yangtze University, 1800 Lihu Road, Wuxi 214122, Jiangsu Province, China.
Waste Manag. 2008 Dec;28(12):2614-22. doi: 10.1016/j.wasman.2008.02.001. Epub 2008 May 27.
The effects of various pretreatment methods, including thermo-acid, thermo-alkaline, ultrasonic-alkaline and ultrasonic-acid, on solubilization and subsequent acidification efficiency of waste activated sludge (WAS) were investigated. The results showed that both thermo-alkaline and ultrasonic-alkaline significantly improved the solubilization of WAS at a high concentration (7.4% of total solids). Solubilization of volatile solids (VS) and crude protein was 60.2-61.6% and 66.8-67.5%, respectively. Thermo-alkaline or ultrasonic-alkaline pretreatment also enhanced the efficiency of subsequent WAS acidification. Experimental results showed that they had similar effects on the acidification of WAS. The conversion ratio from VS to total volatile fatty acids (TVFAs) was 0.230 in the case of the ultrasonic-alkaline pretreatment. Through this pretreatment, the level of TVFAs was 68% more than the untreated WAS after 252 h of fermentation. The mechanism of TVFAs production under different pretreatment methods was further investigated and the results indicated that soluble protein was the main substrate for TVFAs production from the WAS after the thermo-alkaline or ultrasonic-alkaline pretreatment.
研究了包括热酸、热碱、超声碱和超声酸在内的各种预处理方法对废弃活性污泥（WAS）溶解及后续酸化效率的影响。结果表明，热碱和超声碱预处理均显著提高了高浓度（总固体含量的7.4%）WAS的溶解率。挥发性固体（VS）和粗蛋白的溶解率分别为60.2% - 61.6%和66.8% - 67.5%。热碱或超声碱预处理还提高了后续WAS酸化的效率。实验结果表明，它们对WAS酸化具有相似的效果。超声碱预处理条件下，VS向总挥发性脂肪酸（TVFAs）的转化率为0.230。经过这种预处理，发酵252小时后，TVFAs的含量比未处理的WAS高出68%。进一步研究了不同预处理方法下TVFAs的产生机制，结果表明，热碱或超声碱预处理后，可溶性蛋白是WAS产生TVFAs的主要底物。
