Brochet Xavier, Lefranc Marie-Paule, Giudicelli Véronique
IMGT, the international ImMunoGeneTics information system, Laboratoire d'ImmunoGénétique Moléculaire LIGM, Université Montpellier 2, Institut de Génétique Humaine IGH, UPR CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier cedex 5, France.
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W503-8. doi: 10.1093/nar/gkn316. Epub 2008 May 24.
IMGT/V-QUEST is the highly customized and integrated system for the standardized analysis of the immunoglobulin (IG) and T cell receptor (TR) rearranged nucleotide sequences. IMGT/V-QUEST identifies the variable (V), diversity (D) and joining (J) genes and alleles by alignment with the germline IG and TR gene and allele sequences of the IMGT reference directory. New functionalities were added through a complete rewrite in Java. IMGT/V-QUEST analyses batches of sequences (up to 50) in a single run. IMGT/V-QUEST describes the V-REGION mutations and identifies the hot spot positions in the closest germline V gene. IMGT/V-QUEST can detect insertions and deletions in the submitted sequences by reference to the IMGT unique numbering. IMGT/V-QUEST integrates IMGT/JunctionAnalysis for a detailed analysis of the V-J and V-D-J junctions, and IMGT/Automat for a full V-J- and V-D-J-REGION annotation. IMGT/V-QUEST displays, in 'Detailed view', the results and alignments for each submitted sequence individually and, in 'Synthesis view', the alignments of the sequences that, in a given run, express the same V gene and allele. The 'Advanced parameters' allow to modify default parameters used by IMGT/V-QUEST and IMGT/JunctionAnalysis according to the users' interest. IMGT/V-QUEST is freely available for academic research at http://imgt.cines.fr.
IMGT/V-QUEST是用于免疫球蛋白（IG）和T细胞受体（TR）重排核苷酸序列标准化分析的高度定制化集成系统。IMGT/V-QUEST通过与IMGT参考目录中的种系IG和TR基因及等位基因序列比对，识别可变（V）、多样（D）和连接（J）基因及等位基因。通过用Java完全重写添加了新功能。IMGT/V-QUEST单次运行可分析一批序列（最多50个）。IMGT/V-QUEST描述V区突变并识别最接近的种系V基因中的热点位置。IMGT/V-QUEST可通过参考IMGT独特编号检测提交序列中的插入和缺失。IMGT/V-QUEST整合了IMGT/JunctionAnalysis用于对V-J和V-D-J连接进行详细分析，以及IMGT/Automat用于完整的V-J-和V-D-J区注释。IMGT/V-QUEST在“详细视图”中单独显示每个提交序列的结果和比对，在“综合视图”中显示在给定运行中表达相同V基因和等位基因的序列的比对。“高级参数”允许根据用户兴趣修改IMGT/V-QUEST和IMGT/JunctionAnalysis使用的默认参数。IMGT/V-QUEST可在http://imgt.cines.fr上免费用于学术研究。
