Ahrenkiel S P, Yu P R, Murphy J E, Nedeljković J M, Donohoe B S
National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, CO 80401, USA.
J Microsc. 2008 Jun;230(Pt 3):382-7. doi: 10.1111/j.1365-2818.2008.01996.x.
Tomographic reconstruction by transmission electron microscopy is used to reveal three-dimensional nanoparticle shapes and the stacking configurations of nanoparticle ensembles. Reconstructions are generated from bright-field image tilt series, with a sample tilt range up to +/- 70 degrees, using single or dual tilt axes. We demonstrate the feasibility of this technique for the analysis of nanomaterials, using appropriate acquisition conditions. Tomography reveals both cubic and hexagonal close-packing configurations in multi-layered arrays of size-selected In nanospheres. By tomography and phase-contrast lattice imaging, we relate the three-dimensional shape of PbSe octahedral nanoparticles to the underlying crystal structure. We also confirm simple-cubic packing in multi-layers of PbSe nanocubes and see evidence that the particle shapes have cubic symmetry. The shapes of TiO(2) nanorod bundles are shown by tomographic reconstruction to resemble flattened ellipsoids.
透射电子显微镜断层扫描重建技术用于揭示三维纳米颗粒形状以及纳米颗粒聚集体的堆积构型。重建是通过明场图像倾斜序列生成的，样品倾斜范围可达±70度，使用单倾斜轴或双倾斜轴。我们使用适当的采集条件，证明了该技术用于分析纳米材料的可行性。断层扫描揭示了尺寸选择的铟纳米球多层阵列中的立方和六方密堆积构型。通过断层扫描和相衬晶格成像，我们将硒化铅八面体纳米颗粒的三维形状与底层晶体结构联系起来。我们还证实了硒化铅纳米立方体多层中的简单立方堆积，并发现了颗粒形状具有立方对称性的证据。断层扫描重建显示二氧化钛纳米棒束的形状类似于扁平椭球体。
