通过区带超速离心法分离后人类血浆脂蛋白的电子显微镜表征（作者译）

[Electronmicroscopic characterisation of human plasma lipoproteins after isolation by zonal ultracentrifugation (author's transl)].

作者信息

Patsch J R, Sailer S, Braunsteiner H, Forte T

出版信息

Dtsch Med Wochenschr. 1976 Oct 29;101(44):1607-10. doi: 10.1055/s-0028-1104311.

Abstract

Human plasma proteins were isolated by zonal ultracentrifugation and the fractions obtained examined under the electron microscope. HDL2 had a high degree of uniformity in normal persons, characterised by numerous two-dimensional, hexagonal arrangements. Optical diffraction analysis of HDL2 gave a centre distance of 11.3 nm, individual HDL2 particles being spherical with a diameter of 9.8 nm. HDL3 represented a somewhat less uniform lipoprotein population with a particle diameter between 6.5 and 9.5 nm. LDL had a diameter of 20-25 nm. In patients with hyperlipoproteinaemia type III a third peak was isolated, in addition to the two normal lipoprotein peaks VLDL and LDL. It lies between them and has been called Lp III. VLDL from type III plasma consisted of spherical particles, diameter 31-81 nm, morphologically indistinguishable from normal VLDL. Lp III consisted of a relatively homogeneous spherical lipoprotein population with a diameter of 25-30 nm. LDL consisted of spherical particles whose size was highly homogeneous (18-23 nm diameter), indistinguishable from normal LDL.

摘要

通过区带超速离心法分离人血浆蛋白，并对所得组分进行电子显微镜检查。在正常人中，高密度脂蛋白2（HDL2）具有高度的均匀性，其特征是存在大量二维六边形排列。对HDL2进行光学衍射分析得出中心间距为11.3 nm，单个HDL2颗粒呈球形，直径为9.8 nm。高密度脂蛋白3（HDL3）代表的脂蛋白群体均匀性稍低，颗粒直径在6.5至9.5 nm之间。低密度脂蛋白（LDL）直径为20 - 25 nm。在III型高脂蛋白血症患者中，除了两个正常的脂蛋白峰即极低密度脂蛋白（VLDL）和LDL外，还分离出了第三个峰。它位于两者之间，被称为脂蛋白III（Lp III）。III型血浆中的VLDL由球形颗粒组成，直径为31 - 81 nm，在形态上与正常VLDL无法区分。Lp III由直径为25 - 30 nm的相对均匀的球形脂蛋白群体组成。LDL由大小高度均匀（直径18 - 23 nm）的球形颗粒组成，与正常LDL无法区分。