Lozovskiĭ V T, Shmelev G E, Noskin V A, Lapshin E N, Dobretsov G E
Biofizika. 1987 Mar-Apr;32(2):285-91.
Particle diameters of very low density lipoprotein (VLDL)--29.5, 36.3, 22.0; low density lipoprotein (LDL)--18.4, 19.0, 22.0; high density lipoprotein subclass 2 (HDL2)--8.5, 9.7, 15.0; and high density lipoprotein subclass 3 (HDL3)--7.2, 7.6, 14.4 nm were evaluated by means of flotation velocity (FV), optical mixing (OM), and fluorescent probe (FP) respectively. On the basis of the calculated frictional ratio f/f0 from FV and OM data for VLDL, LDL, HDL2, and HDL3--1.51, 1.07, 1.31, and 1.10, assuming the sphericity lipoprotein particles as an index for structural peculiarity a conclusion is made that in contrast to LDL and HDL3 HDL2 have asymmetrical weight distribution per particle volume. A model is suggested suitable for the structural peculiarity of VLDL established on the data of three independent methods which outlines VLDL as particles consisting of several associated subunits with the mean diameter of about 20 nm.
分别通过漂浮速度（FV）、光学混合（OM）和荧光探针（FP）评估极低密度脂蛋白（VLDL）的颗粒直径——29.5、36.3、22.0；低密度脂蛋白（LDL）——18.4、19.0、22.0；高密度脂蛋白亚类2（HDL2）——8.5、9.7、15.0；以及高密度脂蛋白亚类3（HDL3）——7.2、7.6、14.4纳米。根据从VLDL、LDL、HDL2和HDL3的FV和OM数据计算出的摩擦比f/f0——1.51、1.07、1.31和1.10，假设球形脂蛋白颗粒作为结构特殊性的指标，可以得出结论：与LDL和HDL3相比，HDL2在每个颗粒体积上具有不对称的重量分布。提出了一个适用于VLDL结构特殊性的模型，该模型基于三种独立方法的数据建立，将VLDL描绘为由几个相关亚基组成的颗粒，平均直径约为20纳米。
