Danielsson B, Ekman R, Johansson B G
Prep Biochem. 1978;8(4):295-319. doi: 10.1080/00327487808065527.
Procedures for the separation of plasma lipoprotein classes and subclasses by zonal ultracentrifugation are described. The main density classes, very low density lipoproteins (VLDL), low density lipoproteins (LDL) and high density lipoproteins (HDL), in plasma can be separated in a single run for 20 hours. For the isolation of VLDL-LDL a centrifugation time of only 90 minutes is needed. Separations can be performed on plasma volumes varying from 10 to 400 ml in the Ti-14 rotor used; VLDL can in this way be isolated from 400 ml plasma in 30 minutes. The advantages and disadvantages of zonal ultracentrifugation in comparison with the commonly employed differential ultracentrifugation for separation of lipoproteins are discussed.
本文描述了通过区带超速离心法分离血浆脂蛋白类别和亚类的方法。血浆中的主要密度类别，即极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL），可以在单次运行20小时内进行分离。对于分离VLDL-LDL，仅需90分钟的离心时间。使用Ti-14转子时，可以对10至400毫升不等的血浆体积进行分离；通过这种方式，可在30分钟内从400毫升血浆中分离出VLDL。文中还讨论了区带超速离心法与常用的差速超速离心法在分离脂蛋白方面的优缺点。
