人血浆中L-[甲基-¹¹C]蛋氨酸的测定。

Measurement of L-[methyl-11C]methionine in human plasma.

作者信息

Ishiwata K, Kameyama M, Hatazawa J, Kubota K, Ido T

机构信息

Division of Radiopharmaceutical Chemistry, Cyclotron and Radioisotope Center, Tohoku University, Sendai, Japan.

出版信息

Int J Rad Appl Instrum A. 1991;42(1):77-9. doi: 10.1016/0883-2889(91)90127-m.

DOI:10.1016/0883-2889(91)90127-m

PMID:1850396

Abstract

The routine measurement of L-[methyl-11C]methionine in human plasma after i.v. injection of the tracer is described. The protein-free plasma metabolites of the tracer were analyzed using a cation-exchange column (Aminex A-6) eluted with a pH 4.25 citrate buffer. The ratios of L-[methyl-11C]methionine for the total radioactivity decreased gradually, and the standard deviations among 19 humans were relatively small for 30 min: 97.2 +/- 1.4%, 5 min; 92.6 +/- 2.1%, 10 min; 89.4 +/- 4.0%, 15 min; 84.1 +/- 3.1%, 20 min; 73.1 +/- 9.8%, 30 min; 45.0 +/- 16.8%, 60 min. A convenient analysis using a Bond elute SCX cartridge showed slightly larger ratios than those measured by liquid chromatography.

摘要

本文描述了静脉注射示踪剂后人体血浆中L-[甲基-11C]蛋氨酸的常规测量方法。使用阳离子交换柱（Aminex A-6），用pH 4.25的柠檬酸盐缓冲液洗脱，对示踪剂的无蛋白血浆代谢物进行分析。L-[甲基-11C]蛋氨酸占总放射性的比例逐渐下降，19名受试者在30分钟内的标准差相对较小：5分钟时为97.2±1.4%；10分钟时为92.6±2.1%；15分钟时为89.4±4.0%；20分钟时为84.1±3.1%；30分钟时为73.1±9.8%；60分钟时为45.0±16.8%。使用Bond elute SCX柱进行的便捷分析显示的比例略高于液相色谱法测得的比例。