通过自动固相萃取和高效液相色谱法分析人血浆中的L-[甲基-¹¹C]蛋氨酸及其代谢产物。

Analysis of L-[methyl-11C]methionine and metabolites in human plasma by an automated solid-phase extraction and a high-performance liquid chromatographic procedure.

作者信息

Lindner K J, Hartvig P, Akesson C, Tyrefors N, Sundin A, Långström B

机构信息

Uppsala University PET Centre, University Hospital, Sweden.

出版信息

J Chromatogr B Biomed Appl. 1996 Apr 26;679(1-2):13-9. doi: 10.1016/0378-4347(96)00011-4.

DOI:10.1016/0378-4347(96)00011-4

PMID:8998552

Abstract

A fully automated method for separation of L-[methyl-11C]Methionine from metabolites in patient plasma was developed. L-[methyl-11C]Methionine was isolated from plasma by solid-phase extraction (SPE). The radioactivity retained on the SPE column was eluted and injected onto the HPLC system for separation of in vivo formed L-[methyl-11C]methionine radiolabeled metabolites. The yield through the isolation procedure and HPLC analysis was greater than 95% with a precision better than 5% (R.S.D.). The calculated rate of L-[methyl-11C]methionine transport into tumor tissue was markedly different with and without compensation for radiolabeled metabolites in patient plasma.

摘要

开发了一种从患者血浆中的代谢物中分离L-[甲基-11C]蛋氨酸的全自动方法。通过固相萃取（SPE）从血浆中分离L-[甲基-11C]蛋氨酸。保留在SPE柱上的放射性物质被洗脱并注入高效液相色谱系统，以分离体内形成的L-[甲基-11C]蛋氨酸放射性标记代谢物。通过分离程序和高效液相色谱分析的产率大于95%，精密度优于5%（相对标准偏差）。在考虑和不考虑患者血浆中放射性标记代谢物的情况下，计算出的L-[甲基-11C]蛋氨酸向肿瘤组织的转运速率明显不同。