Dharma Shalmali J, Modi Deepak N, Nandedkar Tarala D
National Institute for Research in Reproductive Health, Parel, Mumbai, India.
Fertil Steril. 2009 May;91(5 Suppl):2025-36. doi: 10.1016/j.fertnstert.2008.02.088. Epub 2008 May 27.
To evaluate genes involved in ovarian primordial-to-primary follicle transition.
Experimental animal study.
Research institute.
ANIMAL(S): Day-2 and day-4 female Swiss mice.
INTERVENTION(S): We conducted a complementary DNA array study using ovarian messenger RNAs from day-2 and day-4 mice.
MAIN OUTCOME MEASURE(S): The expression profiles of 1,176 genes in neonatal mouse ovaries on day 2 and day 4, which contain primordial and primary follicles, respectively, were compared.
RESULT(S): Twenty-six percent of genes were differentially expressed between day-2 and day-4 ovaries, with 19% being up-regulated and 7% down-regulated in day 4. Analysis of differentially expressed genes revealed that the primordial-to-primary follicle stage transition is associated with induction in the expression of mainly growth factors, immune-related factors, hormone and hormone receptors, and signal transducers. The transition is also associated with proliferation of granulosa cells and absence of apoptosis. In addition, our studies demonstrated that the primary follicles express estrogen receptor beta and are responsive to estrogen actions in vitro in terms of increase in the number of primary follicles and granulosa cell proliferation.
CONCLUSION(S): The transition of primordial to primary follicles is associated with the participation of multiple pathways in regulating gene expression.
评估参与卵巢原始卵泡向初级卵泡转变的基因。
实验动物研究。
研究所。
2日龄和4日龄雌性瑞士小鼠。
我们使用2日龄和4日龄小鼠的卵巢信使核糖核酸进行了互补脱氧核糖核酸阵列研究。
比较了分别含有原始卵泡和初级卵泡的2日龄和4日龄新生小鼠卵巢中1176个基因的表达谱。
26%的基因在2日龄和4日龄卵巢之间存在差异表达，其中4日龄时19%上调，7%下调。对差异表达基因的分析表明，原始卵泡向初级卵泡阶段的转变主要与生长因子、免疫相关因子、激素和激素受体以及信号转导分子的表达诱导有关。这种转变还与颗粒细胞的增殖和凋亡缺失有关。此外，我们的研究表明，初级卵泡表达雌激素受体β，并且在体外对雌激素作用有反应，表现为初级卵泡数量增加和颗粒细胞增殖。
原始卵泡向初级卵泡的转变与多种调节基因表达的途径参与有关。
