Gómez Daniel, Espinosa Elena, Bertazzo Marcelo, Lucas-Elío Patricia, Solano Francisco, Sanchez-Amat Antonio
Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, Murcia 30100, Spain.
Appl Microbiol Biotechnol. 2008 Jul;79(6):925-30. doi: 10.1007/s00253-008-1499-x. Epub 2008 May 27.
Two purple pigmented bacterial strains, CPMOR-1 and CPMOR-2, have been newly isolated from the Mediterranean Sea. 16S RNA sequencing and phenotypic characteristics indicate that they belong to the species Pseudoalteromonas luteoviolacea. The synthesis of macromolecules with antimicrobial activity is a capacity described in many strains of this species although the nature of those macromolecules has not been reported up to now. The search for antimicrobial compounds in the two new strains described in this work shows that they synthesize a macromolecule with antimicrobial activity that can be inhibited by catalase, as it had been described in the type strain P. luteoviolacea NCIMB 1893(T). This work elucidates the nature of such macromolecule as a novel L-amino acid oxidase (LAO) with broad substrate specificity. The enzyme is most active with Met, Gln, Leu, Phe, Glu, and Trp. In growth media containing those amino acids, the hydrogen peroxide generated by the reaction catalyzed by the LAO mediates its antimicrobial activity.
从地中海新分离出两株紫色色素细菌菌株CPMOR-1和CPMOR-2。16S RNA测序和表型特征表明它们属于 luteoviolacea 假交替单胞菌物种。具有抗菌活性的大分子合成是该物种许多菌株所具有的一种能力，尽管迄今为止尚未报道这些大分子的性质。对本研究中描述的两株新菌株中的抗菌化合物进行的研究表明，它们合成了一种具有抗菌活性的大分子，该大分子可被过氧化氢酶抑制，正如在模式菌株 luteoviolacea 假交替单胞菌NCIMB 1893(T)中所描述的那样。这项工作阐明了这种大分子的性质是一种具有广泛底物特异性的新型L-氨基酸氧化酶（LAO）。该酶对蛋氨酸、谷氨酰胺、亮氨酸、苯丙氨酸、谷氨酸和色氨酸的活性最高。在含有这些氨基酸的生长培养基中，由LAO催化的反应产生的过氧化氢介导其抗菌活性。
