保加利亚蜂胶的化学成分与抗菌活性研究

A study of chemical composition and antimicrobial activity of Bulgarian propolis.

作者信息

Gardjeva Petya A, Dimitrova Stela Z, Kostadinov Iliya D, Murdjeva Marianna A, Peyche Lyudmil P, Lukanov Lyudmil K, Stanimirova Irina V, Alexandrov Alexandar S

机构信息

Department of Microbiology and Immunology, ' Medical University, Plovdiv, Bulgaria.

出版信息

Folia Med (Plovdiv). 2007;49(3-4):63-9.

PMID:18504937

Abstract

UNLABELLED

Propolis is a natural product which exhibits pronounced antimicrobial and anti-inflammatory effects.

AIM

To analyze the chemical composition and antimicrobial effect of Bulgarian propolis, p-cumaric acid and ferulic acid on certain Gram-positive and Gram-negative microorganisms.

MATERIAL AND METHODS

Ethanol extract of Bulgarian propolis (Propolin) and water extract of Bulgarian propolis (Propolstom), ferulic acid and p-cumaric acid were used. The chemical HPLC-analysis of propolis was performed with Varian Prostar system. Microbiological tests were done on isolates of Streptococcus beta-haemolyticus, Enterococcus faecalis, Candida albicans and Escherichia coli. Non-treated and ethanol-treated cultures of the corresponding strains were used as controls. Minimal bactericidal and bacteriostatic concentration of Propolin were determined by agar method.

RESULTS

The concentration of p-cumaric acid and ferulic acid in ethanol extract of propolis is about 1%. The minimal bactericidal concentration of Bulgarian propolis against Streptococcus beta-haemolyticus is 281 +/- 89.3 microg/ml, against Enterococcus faecalis--3490 +/- 633.1 microg/ml, against Candida albicans--1375 +/- 255.2 microg/ml, and against Escherichia coli--24306 +/- 1706.4 microg/ml. The minimal bactericidal concentration of p-cumaric acid and ferulic acid against Streptococcus beta-haemolyticus is 212 +/- 6.5 microg/ml and 208 +/- 7.7 microg/ml, against Enterococcus faecalis--3013 +/- 176.6 and 3032 +/- 203.4 microg/ml, against Candida albicans--157 +/- 15.5 microg/ml and 149 +/- 23.2 microg/ml, and against Escherichia coli--798 +/- 7.7 microg/ml and 801 +/- 22.8 microg/ml, respectively.

CONCLUSIONS

The phenolic compounds p-cumaric acid and ferulic acid are 10 times less extracted in water extraction than in ethanol extraction. The most susceptible strains to the Bulgarian propolis and phenolic compounds are Streptococcus beta-haemolyticus and Candida albicans and the least affected are Escherichia coli and Enterococcus faecalis.

摘要

未标注

蜂胶是一种具有显著抗菌和抗炎作用的天然产物。

目的

分析保加利亚蜂胶、对香豆酸和阿魏酸对某些革兰氏阳性和革兰氏阴性微生物的化学成分及抗菌效果。

材料与方法

使用保加利亚蜂胶乙醇提取物（Propolin）、保加利亚蜂胶水提取物（Propolstom）、阿魏酸和对香豆酸。蜂胶的化学高效液相色谱分析采用瓦里安Prostar系统进行。对β - 溶血性链球菌、粪肠球菌、白色念珠菌和大肠杆菌的分离株进行微生物学试验。相应菌株的未处理和乙醇处理培养物用作对照。通过琼脂法测定Propolin的最低杀菌浓度和抑菌浓度。

结果

蜂胶乙醇提取物中对香豆酸和阿魏酸的浓度约为1%。保加利亚蜂胶对β - 溶血性链球菌的最低杀菌浓度为281±89.3微克/毫升，对粪肠球菌为3490±633.1微克/毫升，对白色念珠菌为1375±255.2微克/毫升，对大肠杆菌为24306±1706.4微克/毫升。对香豆酸和阿魏酸对β - 溶血性链球菌的最低杀菌浓度分别为212±6.5微克/毫升和208±7.7微克/毫升，对粪肠球菌为3013±176.6和3032±203.4微克/毫升，对白色念珠菌为157±15.5微克/毫升和149±23.2微克/毫升，对大肠杆菌分别为798±7.7微克/毫升和801±22.8微克/毫升。