Department of Pharmaceutical Technology and Social Pharmacy, Kaunas University of Medicine, A. Mickevičiaus 9, Kaunas 44307, Lithuania.
Nat Prod Res. 2011 Sep;25(15):1463-8. doi: 10.1080/14786419.2010.529440. Epub 2011 Jun 30.
Designing propolis products for external use involves determining the optimal form of propolis for the introduction into dermatological pharmaceuticals and cosmetic preparations. As a potent ingredient, propolis oil extract from raw material harvested in Lithuania was analysed. The rheological characteristics, content of phenolic compounds, major compounds and antimicrobial activity of the propolis oil extract are investigated here for the first time. The propolis oil extract was produced by maceration using different solvents, raw material was collected in Lithuania. Solvent mixture with 96% ethanol increased the rheological stability and extracted amount of phenolic compound. High-performance liquid chromatography identified the potent quality markers for Lithuanian propolis, phenylpropanoid vanillin, coumaric acid and ferulic acid. Antimicrobial activity of propolis oil extract was evaluated in experimental studies in vitro, and the minimal concentration of phenolic compounds that inhibited respective microorganisms was determined. The results demonstrate that phenolic compounds have effective antimicrobial activity in propolis oil extract; thus, it can be compatible with the semisolid preparation.
设计外用蜂胶产品涉及确定将蜂胶引入到皮肤科药物和化妆品制剂中的最佳形式。作为一种有效的成分，分析了从立陶宛收获的原料中提取的蜂胶油提取物。本文首次研究了蜂胶油提取物的流变特性、酚类化合物含量、主要化合物和抗菌活性。蜂胶油提取物通过浸渍使用不同的溶剂生产，原料在立陶宛收集。含有 96%乙醇的溶剂混合物增加了流变稳定性和酚类化合物的提取量。高效液相色谱法确定了立陶宛蜂胶的强效质量标志物，苯丙素香草醛、香豆酸和阿魏酸。在体外实验研究中评估了蜂胶油提取物的抗菌活性，并确定了抑制相应微生物的最小酚类化合物浓度。结果表明，酚类化合物在蜂胶油提取物中具有有效的抗菌活性；因此，它可以与半固体制剂相容。
