Arslan Soley, Ozbilge Hatice, Kaya Esma G, Er Ozgur
Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Erciyes, Talas, Kayseri 38039, Turkey.
Saudi Med J. 2011 May;32(5):479-83.
To evaluate the antimicrobial effect by measuring the minimum inhibitory concentration MIC and minimum bactericidal concentration MBC of propolis, BioPure MTAD, 5% sodium hypochlorite (NaOCl), and 2% chlorhexidine CHX on Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) in vitro.
This study was performed in the Faculty of Dentistry and Pharmacy at Erciyes University, Kayseri, Turkey from February to April 2010. Ethanol extract of propolis (EEP) was prepared from propolis collected from Kayseri, Turkey, and proper media for microorganisms were prepared using sterile broth medium to give final concentrations between 0.002-2.4 mg/ml for propolis, 0.000125-0.512 mg/ml for CHX, and 1:2-1:4096 dilutions for NaOCl and BioPure MTAD. Using the macrobroth dilution method, MIC, and MBC values of irrigants on the growth of E. faecalis and C. albicans were determined.
Propolis and other irrigants were found to be effective on C. albicans and E. faecalis. Propolis and NaOCl were more effective in lower concentrations on C. albicans than on E. faecalis. In contrast, CHX and MTAD were more effective in lower concentrations on E. faecalis than on C. albicans.
Propolis showed antimicrobial activity against E. faecalis and C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans.
通过测量蜂胶、BioPure MTAD、5%次氯酸钠（NaOCl）和2%氯己定（CHX）对粪肠球菌（E. faecalis）和白色念珠菌（C. albicans）的最低抑菌浓度（MIC）和最低杀菌浓度（MBC），评估其体外抗菌效果。
本研究于2010年2月至4月在土耳其开塞利埃尔西耶斯大学牙科学院和药学院进行。从土耳其开塞利采集的蜂胶制备乙醇提取物（EEP），并使用无菌肉汤培养基制备适合微生物生长的培养基，使蜂胶的最终浓度为0.002 - 2.4 mg/ml，CHX为0.000125 - 0.512 mg/ml，NaOCl和BioPure MTAD为1:2 - 1:4096稀释度。采用常量肉汤稀释法，测定冲洗剂对粪肠球菌和白色念珠菌生长的MIC和MBC值。
发现蜂胶和其他冲洗剂对白色念珠菌和粪肠球菌有效。蜂胶和NaOCl在较低浓度下对白色念珠菌的效果比对粪肠球菌更好。相比之下，CHX和MTAD在较低浓度下对粪肠球菌的效果比对白色念珠菌更好。
蜂胶对粪肠球菌和白色念珠菌具有抗菌活性。看来蜂胶是根除粪肠球菌和白色念珠菌的一种有效的根管冲洗剂。
