Colas C, Popot M-A, Garcia P, Bonnaire Y, Bouchonnet S
Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370 Verrières le Buisson, France.
Biomed Chromatogr. 2008 Aug;22(8):912-7. doi: 10.1002/bmc.1030.
LC/ESI-MS n methods have been previously set up to detect the administration of (i) Harpagophytum and (ii) preparations containing a plant capable of anti-stress properties: Eleutherococcus senticosus. Harpagoside has been found to be the main indicator of Harpagophytum administration in the horse. These methods have been applied to a large number of horse urine samples of various origins. Regarding the detection of Harpagophytum administration, harpagoside, harpagide and 8-para-coumaroyl harpagide were detected together in only one sample out of 317. Eleutheroside E was found to be the main indicator of Eleutherococcus senticosus administration. It was detected in post-administration samples collected from two horses having received a feed supplement containing Eleutherococcus senticosus for several days. Out of the 382 samples tested, eleutheroside E was found in an unexpected large number of urine samples (39%) of various origins and its presence cannot be only due to the sole use of herbal dietary supplements.
液相色谱/电喷雾电离质谱联用（LC/ESI-MS n）方法先前已被建立，用于检测（i）南非钩麻以及（ii）含有具有抗应激特性植物（刺五加）的制剂的施用情况。哈帕苷已被发现是马体内施用南非钩麻的主要指标。这些方法已应用于大量不同来源的马尿样本。关于南非钩麻施用情况的检测，在317个样本中，仅有1个样本同时检测到了哈帕苷、哈帕吉德和8-对香豆酰哈帕吉德。刺五加苷E被发现是刺五加施用的主要指标。在从两匹连续几天食用含有刺五加的饲料补充剂的马身上采集的给药后样本中检测到了该物质。在测试的382个样本中，在大量不同来源的意外尿液样本（39%）中发现了刺五加苷E，其存在不能仅归因于草药膳食补充剂的单独使用。
