Murthy Hosakatte Niranjana, Kim Yun-Soo, Georgiev Milen I, Paek Kee-Yoeup
Research Center for the Development Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea,
Appl Microbiol Biotechnol. 2014 Sep;98(17):7319-29. doi: 10.1007/s00253-014-5899-9. Epub 2014 Jul 9.
Eleutherosides, the phenylpropanoid and lignan glycosides, are the active ingredients accumulated in the roots and stems of Eleutherococcus species and in Eleutherococcus senticosus in particular. Syringin (=eleutheroside B) and (-) syringaresinol-di-O-β-D-glucoside (=eleutheroside E) appear as the most important bioactive compounds which are used as adaptogens, besides their abundant antidiabetic and anticancer properties. As the availability of "Eleuthero" is becoming increasingly limited because of its scanty natural distribution, the production of these compounds by biotechnological means has become an attractive alternative. In E. senticosus and other closely related species, Eleutherococcus sessiliflorus, Eleutherococcus chiisanensis, and Eleutherococcus koreanum, organogenic cultures have been induced for the production of eleutherosides. Bioreactor cultures have been established and various parameters, which influence on the accumulation of biomass and secondary metabolites, have been thoroughly investigated. Pilot-scale cultures have also been accomplished for the large-scale production of somatic embryos containing abundant amounts of eleutherosides. This review describes the biotechnological approaches and challenges for the production of eleutherosides.
刺五加苷，即苯丙素和木脂素糖苷，是刺五加属植物，特别是刺五加的根和茎中积累的活性成分。丁香苷（=刺五加苷B）和（-）丁香树脂醇二-O-β-D-葡萄糖苷（=刺五加苷E）是最重要的生物活性化合物，除了具有丰富的抗糖尿病和抗癌特性外，还被用作适应原。由于“刺五加”的自然分布稀少，其可用性日益受限，因此通过生物技术手段生产这些化合物已成为一种有吸引力的替代方法。在刺五加以及其他近缘物种，如无梗五加、朝鲜刺五加和毛梗刺五加中，已诱导出器官发生培养物用于生产刺五加苷。已建立生物反应器培养，并对影响生物量和次生代谢产物积累的各种参数进行了深入研究。还完成了中试规模的培养，用于大规模生产含有大量刺五加苷的体细胞胚。本综述描述了生产刺五加苷的生物技术方法和挑战。
