Rehman Abdul, Farooq Hina, Hasnain Shahida
Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan.
J Basic Microbiol. 2008 Jun;48(3):195-201. doi: 10.1002/jobm.200700324.
The present study is aimed at assessing the ability of metal resistant yeast, Loddermyces elongisporus, to uptake metal from liquid medium. The minimum inhibitory concentration of Cu(2+) against Loddermyces elongisporus ranged between 2.2-2.3 mg/l. The yeast could also tolerate Zn(2+) (2.9 mg/l), Hg(2+) (2.4 mg/l), Ni(2+) (2.2 mg/l), Cr(6+) (2.0 mg/l), Pb(2+) (1.1 mg/l), and Cd(2+) (0.8 mg/l). The yeast isolate showed typical growth curves but lag and log phases extended in the presence of copper. Yeast isolate showed optimum growth at 30 degrees C and pH 8. Metal processing ability of the isolate was determined in a medium containing 0.1 mg/l of Cu(2+). Loddermyces elongisporus could reduce Cu(2+) 15%, 26%, 39%, 50%, 60%, 67%, 75% and 81% from the medium after 6, 12, 18, 24, 30, 48, 72 and 96 hours, respectively. L. elongisporus could also efficiently remove 80% copper from the medium after 96 h and was able to remove Cu(2+) 60% and 77% from the wastewater after 4 and 8 d, respectively. The metal binding ability suggests possibility of using this yeast strain for removal of copper from metal contaminated wastewater.
本研究旨在评估耐金属酵母——长孢洛德酵母从液体培养基中摄取金属的能力。铜离子（Cu(2+)）对长孢洛德酵母的最低抑菌浓度在2.2 - 2.3毫克/升之间。该酵母还能耐受锌离子（Zn(2+)，2.9毫克/升）、汞离子（Hg(2+)，2.4毫克/升）、镍离子（Ni(2+)，2.2毫克/升）、六价铬（Cr(6+)，2.0毫克/升）、铅离子（Pb(2+)，1.1毫克/升）和镉离子（Cd(2+)，0.8毫克/升）。该酵母分离株呈现出典型的生长曲线，但在有铜存在的情况下，延迟期和对数期会延长。酵母分离株在30摄氏度和pH值为8时生长最佳。在含有0.1毫克/升铜离子的培养基中测定了该分离株的金属处理能力。长孢洛德酵母在6、12、18、24、30、48、72和96小时后，分别能从培养基中还原15%、26%、39%、50%、60%、67%、75%和81%的铜离子。96小时后，长孢洛德酵母还能有效地从培养基中去除80%的铜，并且在4天和8天后，分别能从废水中去除60%和77%的铜离子。这种金属结合能力表明该酵母菌株有可能用于从金属污染废水中去除铜。
