大鼠非酒精性脂肪性肝炎进展过程中肠黏膜屏障功能的变化
Change of intestinal mucosa barrier function in the progress of non-alcoholic steatohepatitis in rats.
作者信息
Li Sheng, Wu Wan-Chun, He Chi-Yi, Han Zhen, Jin Dao-You, Wang Lin
机构信息
Department of Gastroenterology, Yijishan Hospital, Wuhu 241001, Anhui Province, China.
出版信息
World J Gastroenterol. 2008 May 28;14(20):3254-8. doi: 10.3748/wjg.14.3254.
AIM
To explore the change of intestinal mucosa barrier function in the progress of non-alcoholic steatohepatitis (NASH) in rats.
METHODS
Thirty-two Sprague-Dawley (SD) rats were randomly divided into control group and model group. Rats in the control group were given normal diet, and rats in the model group were given fat-rich diet. Eight rats in each group were killed at end of the 8th and 12th wk, respectively. The levels of endotoxin, D-xylose, TG, TC, ALT and AST, intestinal tissue SOD and MDA as well as intestinal mucus secretory IgA (sIgA) were measured. The pathology of liver was observed by HE staining.
RESULTS
At end of the 8th wk, there was no marked difference in the levels of endotoxin, D-xylose and sIgA between the two groups. At end of the 12th wk, rats in the model group developed steatohepatitis and had a higher serum level of endotoxin (P = 0.01) and D-xylose (P = 0.00) and a lower serum level of sIgA (P = 0.007).
CONCLUSION
Intestinal mucosa barrier malfunction may exist in NASH rats and may be an important promoter of NASH in rats.
目的
探讨大鼠非酒精性脂肪性肝炎（NASH）进程中肠黏膜屏障功能的变化。
方法
将32只Sprague-Dawley（SD）大鼠随机分为对照组和模型组。对照组大鼠给予正常饮食，模型组大鼠给予高脂饮食。每组分别于第8周和第12周结束时处死8只大鼠。检测内毒素、D-木糖、甘油三酯（TG）、总胆固醇（TC）、谷丙转氨酶（ALT）和谷草转氨酶（AST）水平、肠组织超氧化物歧化酶（SOD）和丙二醛（MDA）以及肠黏液分泌型免疫球蛋白A（sIgA）。通过苏木精-伊红（HE）染色观察肝脏病理。
结果
第8周结束时，两组内毒素、D-木糖和sIgA水平无明显差异。第12周结束时，模型组大鼠发生脂肪性肝炎，血清内毒素水平较高（P = 0.01），D-木糖水平较高（P = 0.00），血清sIgA水平较低（P = 0.007）。
结论
NASH大鼠可能存在肠黏膜屏障功能障碍，这可能是大鼠NASH的重要促进因素。
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