巴西人群中多态性异生物质代谢酶基因型与口腔癌发生及进展之间缺乏关联。
Lack of association among polymorphic xenobiotic-metabolizing enzyme genotypes and the occurrence and progression of oral carcinoma in a Brazilian population.
作者信息
Losi-Guembarovski Roberta, Cólus Ilce Mara de Syllos, De Menezes Rodrigo Paes, Poliseli Fernando, Chaves Vivian Nappi, Kuasne Hellen, Leichsenring Andrei, Guembarovski Alda Losi, Oliveira Benedito W, Ramos Gyl, Cavalcanti Teresa C S, Mizuno Lauro Toyshi, Cavalli Iglenir João, Ribeiro Enilze Maria de Souza Fonseca
机构信息
Laboratório de Citogenética Humana e Oncogenética, Universidade Federal do Paraná, Curitiba, PR, Brazil.
出版信息
Anticancer Res. 2008 Mar-Apr;28(2A):1023-8.
BACKGROUND
A case control association study was carried out to investigate polymorphisms in genes CYP1A1 (3801T > C), GSTM1, and GSTT1 (null genotypes) and oral squamous cell carcinoma (OSCC), including a correlation with some histopathological findings (tumor size, lymph node invasion and degree of tumor differentiation).
PATIENTS AND METHODS
The patients (n = 91) and the controls (n = 81) were matched by age, sex, ethnicity and smoking habits. The molecular analysis was carried out using Polymerase Chain Reaction-Restrict Length Polymorphisms PCR-RFLP (CYP1A1) and Multiplex-PCR (GSTM1/GSTT1).
RESULTS
No association was found for any of the studied genes: CYP1A1 (odds ratio (OR) = 1.24; 95% Confidence Interval (CI) = 0.67-2.31), GSTM1 (OR = 0.61; CI 95% = 0.33-1.11), and GSTT1 (OR = 1.24; CI 95% = 0.65-2.38). The analysis of combining genotypes also showed lack of association. Comparison with the histopathological findings did not, in general, detect any statistically significant differences.
CONCLUSION
CYP1A1, GSTM1 and GSTT1 polymorphisms do not appear to influence the genetic susceptibility to OSCC or the progression to more advanced stages.
背景
开展了一项病例对照关联研究，以调查CYP1A1基因（3801T>C）、GSTM1基因和GSTT1基因（无效基因型）的多态性与口腔鳞状细胞癌（OSCC）之间的关系，包括与一些组织病理学结果（肿瘤大小、淋巴结浸润和肿瘤分化程度）的相关性。
患者和方法
患者（n=91）和对照组（n=81）按年龄、性别、种族和吸烟习惯进行匹配。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）（用于CYP1A1基因）和多重PCR（用于GSTM1/GSTT1基因）进行分子分析。
结果
在所研究的任何基因中均未发现关联：CYP1A1基因（优势比（OR）=1.24；95%置信区间（CI）=0.67-2.31）、GSTM1基因（OR=0.61；95%CI=0.33-1.11）和GSTT1基因（OR=1.24；95%CI=0.65-2.38）。联合基因型分析也显示无关联。与组织病理学结果的比较总体上未发现任何统计学上的显著差异。
结论
CYP1A1、GSTM1和GSTT1基因多态性似乎不会影响OSCC的遗传易感性或进展至更晚期阶段。
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