Turton Jane F, Baklan Hatice, Siu L K, Kaufmann Mary E, Pitt Tyrone L
Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London, UK.
FEMS Microbiol Lett. 2008 Jul;284(2):247-52. doi: 10.1111/j.1574-6968.2008.01208.x. Epub 2008 May 27.
A multiplex PCR using targets within the serotype-specific region of the capsular polysaccharide synthesis gene cluster of serotypes K1, K2 and K5 was evaluated using the 77 reference serotype strains of Klebsiella, and a panel of clinical isolates subjected previously to conventional serotyping. The PCR was highly specific for these serotypes, which are those most associated with virulence in humans and horses. PCR confirmed that isolates of the K5 serotype had cross-reacted with antiserum for other serotypes, particularly for K7. K5 isolates received by our laboratory were almost exclusively from thoroughbred horses, and were submitted for screening prior to breeding programmes. Most, including a reference strain isolated in 1955, belonged to a cluster of genetically similar isolates of sequence type (ST) 60. K1 isolates, all from humans, belonged to a previously identified cluster of ST 23.
使用肺炎克雷伯菌的77株参考血清型菌株以及一组先前进行过传统血清分型的临床分离株，对一种多重PCR方法进行了评估，该方法针对血清型K1、K2和K5的荚膜多糖合成基因簇血清型特异性区域内的靶点。该PCR对这些血清型具有高度特异性，这些血清型与人类和马匹的毒力最为相关。PCR证实，K5血清型的分离株与其他血清型的抗血清发生了交叉反应，尤其是与K7血清型。我们实验室收到的K5分离株几乎全部来自纯种马，并且是在繁殖计划之前提交进行筛查的。大多数分离株，包括1955年分离的一株参考菌株，属于序列型（ST）60的一组基因相似的分离株。所有来自人类的K1分离株属于先前鉴定的ST 23簇。
