Seguí-Simarro José M, Nuez Fernando
Instituto para la Conservación y Mejora de la Agrodiversidad Valenciana, Universidad Politécnica de Valencia, Ciudad Politécnica de la Innovación, Valencia, Spain.
Physiol Plant. 2008 Sep;134(1):1-12. doi: 10.1111/j.1399-3054.2008.01113.x. Epub 2008 Apr 11.
Microspore embryogenesis is the most powerful androgenic pathway to produce haploid and doubled haploid plants. To deviate a microspore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the microspore undergoes numerous changes at different levels, from overall morphology to gene expression. Induction of microspore embryogenesis not only implies the expression of an embryogenic program, but also a stress-related cellular response and a repression of the gametophytic program to revert the microspore to a totipotent status. In this review, we compile the most recent advances in the understanding of the changes undergone by the induced microspore to readapt to the new developmental scenario. We devote special attention to the efforts made to uncover changes in the transcriptome of the induced microspore and microspore-derived embryo (MDE). Finally, we discuss the influence that an in vitro environment exerts over the MDE, as compared with its zygotic counterpart.
小孢子胚胎发生是产生单倍体和双单倍体植物最有效的雄核发育途径。为了使小孢子转向胚胎发生，许多因素（因物种而异）必须在同一时间和地点共同作用。一旦被诱导，小孢子会在不同水平上经历众多变化，从整体形态到基因表达。小孢子胚胎发生的诱导不仅意味着胚胎发生程序的表达，还意味着与应激相关的细胞反应以及配子体程序的抑制，以使小孢子恢复到全能状态。在本综述中，我们汇总了在理解诱导小孢子为适应新的发育情况而经历的变化方面的最新进展。我们特别关注为揭示诱导小孢子和小孢子衍生胚（MDE）转录组变化所做的努力。最后，我们讨论了体外环境与其合子对应物相比对MDE产生的影响。
