Liu Che, Luo Defang, Streit Wolfgang J, Harrison Jeffrey K
Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, FL 32610-0267, USA.
J Neuroimmunol. 2008 Jul 31;198(1-2):98-105. doi: 10.1016/j.jneuroim.2008.04.016. Epub 2008 May 27.
Human glioblastoma multiforme (GBM) is the most malignant form of human brain tumors. A characteristic of GBM is the marked presence of tumor infiltrated microglia/macrophages and lymphocytes. The goal of this study was directed toward understanding the role of the chemokine system CX3CL1 and its receptor CX3CR1 in the GL261 murine model of malignant glioma. In situ hybridization analysis identified CX3CL1 and CX3CR1 expression in GL261 tumors. The impact of CX3CR1 deletion on the growth of intracranial GL261 gliomas and associated immune cell infiltration was evaluated in CX3CR1 gene-disrupted C57BL/6 mice. A slight increase in the tumor growth rate in CX3CR1-/- mice was evident with similar numbers of microglia and CD4+, CD8+, FoxP3+, or Ly49G2+ lymphocytes within tumors established in CX3CR1 +/- and -/- mice. These data indicate that CX3CR1 has little or no effects on either gliomagenesis or the migration of microglia and lymphocytes into GL261 tumors.
人类多形性胶质母细胞瘤（GBM）是人类脑肿瘤中最恶性的形式。GBM的一个特征是肿瘤浸润的小胶质细胞/巨噬细胞和淋巴细胞明显存在。本研究的目的是了解趋化因子系统CX3CL1及其受体CX3CR1在恶性胶质瘤GL261小鼠模型中的作用。原位杂交分析确定了GL261肿瘤中CX3CL1和CX3CR1的表达。在CX3CR1基因敲除的C57BL/6小鼠中评估了CX3CR1缺失对颅内GL261胶质瘤生长及相关免疫细胞浸润的影响。CX3CR1-/-小鼠的肿瘤生长速率略有增加，在CX3CR1+/-和-/-小鼠中建立的肿瘤内，小胶质细胞以及CD4+、CD8+、FoxP3+或Ly49G2+淋巴细胞数量相似。这些数据表明，CX3CR1对胶质瘤发生或小胶质细胞及淋巴细胞向GL261肿瘤的迁移几乎没有影响。
