Duran R, Ranchou-Peyruse M, Menuet V, Monperrus M, Bareille G, Goñi M S, Salvado J C, Amouroux D, Guyoneaud R, Donard O F X, Caumette P
Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux, UMR CNRS 5254, Université de Pau et des Pays de l'Adour, Avenue de l'Universite, IBEAS BP1155, 64013 Pau Cedex, France.
Environ Pollut. 2008 Dec;156(3):951-8. doi: 10.1016/j.envpol.2008.05.007. Epub 2008 May 27.
In order to study the influence of microorganisms on the mercury biogeochemistry, the metal content and the structure of microbial communities were determined in sediments from stations along the Adour Estuary. The comparison of the bacterial communities and their distribution in function of the environmental parameters by Canonical Correspondence Analysis (CCA) revealed the influence of metals on the bacterial communities structure. Sediments where the bacterial communities are mostly influenced by methylmercury were incubated in slurries with or without mercury, under oxic and anoxic conditions. Methylmercury production was detected in the anoxic biotic slurries with a net methylation yield of 0.3% after 24 h. CCA based on T-RFLP profiles revealed the impact of mercury addition on the bacterial communities structure. In addition, 17 bacterial strains, mainly sulphate-reducing bacteria involved in mercury methylation, were isolated and identified.
为了研究微生物对汞生物地球化学的影响，对阿杜尔河口沿线站点沉积物中的金属含量和微生物群落结构进行了测定。通过典范对应分析（CCA）对细菌群落及其在环境参数作用下的分布进行比较，揭示了金属对细菌群落结构的影响。将细菌群落主要受甲基汞影响的沉积物在有氧和无氧条件下，分别置于含汞或不含汞的泥浆中进行培养。在无氧生物泥浆中检测到了甲基汞的产生，24小时后净甲基化产率为0.3%。基于T-RFLP图谱的CCA揭示了添加汞对细菌群落结构的影响。此外，还分离并鉴定了17株细菌菌株，主要是参与汞甲基化的硫酸盐还原菌。
