Aydoğan Müfide, Korkmaz Asli, Barlas Nurhayat, Kolankaya Dürdane
Department of Biology, Faculty of Science, University of Hacettepe, Ankara, Turkey.
Toxicology. 2008 Jul 10;249(1):35-9. doi: 10.1016/j.tox.2008.04.002. Epub 2008 Apr 12.
Bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) are endocrine-disrupting chemicals that has been shown to exert both toxic and estrogenic effects on mammalian cells. The aim of this study was to investigate if BPA, NP and OP induce oxidative stress on the brain tissue of male rats and if co-administration of vitamin C, an antioxidant, can prevent any possible oxidative stress. The male rats were divided into seven groups as control (vehicle), BPA, NP, OP, BPA+C, NP+C, OP+C. BPA, OP and NP (25 mg/(kg day)) were administrated orally to male Wistar rats for 45 days. In vitamin C co-administration groups (BPA+C, NP+C, OP+C), vitamin C (60 mg/(kg day)) were administrated orally along with BPA, OP and NP (25 mg/(kg day)) treatments. The rats in the control group received olive oil orally. The final body and absolute organ weights of treated rats did not show any significant difference when compared with the control group. Also, there were no significant difference in relative organ weights of BPA, NP, OP, BPA+C and NP+C groups when compared with control group. Only, relative organ weights were increased significantly in OP+C group compared with control group. Decreased levels of reduced glutathione (GSH) were found in the brains of BPA, NP, OP treated rats. The end product of lipid peroxidation, malondialdehyde (MDA), appeared at significantly higher concentrations in the BPA, NP, and OP treated groups when compared to the control group. On the other hand, there were no changes in the brain MDA and GSH levels of BPA+C, NP+C and OP+C groups compared with BPA, NP and OP treatment groups, respectively. In histopathologic examination, the vitamin C co-administrated groups had much more hyperchromatic cells in the brain cortex than that observed in the groups treated with only BPA, NP, and OP. The results of this study demonstrate that BPA, NP and OP generate reactive oxygen species that caused oxidative damage in the brain of male rats. In addition, vitamin C co-administration along with BPA, NP, and OP aggravates this oxidative damage in the brain of rats.
双酚A（BPA）、壬基酚（NP）和辛基酚（OP）是内分泌干扰化学物质，已被证明对哺乳动物细胞具有毒性和雌激素效应。本研究的目的是调查BPA、NP和OP是否会在雄性大鼠的脑组织上诱导氧化应激，以及抗氧化剂维生素C的共同给药是否可以预防任何可能的氧化应激。雄性大鼠被分为七组，即对照组（赋形剂）、BPA组、NP组、OP组、BPA+C组、NP+C组、OP+C组。将BPA、OP和NP（25毫克/（千克·天））口服给予雄性Wistar大鼠，持续45天。在维生素C共同给药组（BPA+C组、NP+C组、OP+C组）中，则将维生素C（60毫克/（千克·天））与BPA、OP和NP（25毫克/（千克·天））一起口服给药。对照组的大鼠口服橄榄油。与对照组相比，经处理的大鼠的最终体重和绝对器官重量没有显示出任何显著差异。此外，与对照组相比，BPA组、NP组、OP组、BPA+C组和NP+C组的相对器官重量也没有显著差异。只有OP+C组的相对器官重量与对照组相比显著增加。在BPA、NP、OP处理的大鼠大脑中发现还原型谷胱甘肽（GSH）水平降低。与对照组相比，脂质过氧化的终产物丙二醛（MDA）在BPA组、NP组和OP组中的浓度显著更高。另一方面，与BPA组、NP组和OP处理组相比，BPA+C组、NP+C组和OP+C组的大脑MDA和GSH水平没有变化。在组织病理学检查中，维生素C共同给药组大脑皮层中的深染细胞比仅用BPA、NP和OP处理的组更多。本研究结果表明，BPA、NP和OP会产生活性氧，从而在雄性大鼠大脑中造成氧化损伤。此外，维生素C与BPA、NP和OP共同给药会加剧大鼠大脑中的这种氧化损伤。
