Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, 09016, Isıklı, Aydın, Turkey.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyon, Turkey.
Biol Trace Elem Res. 2021 Aug;199(8):3001-3012. doi: 10.1007/s12011-020-02418-y. Epub 2020 Oct 7.
This study was aimed at evaluating the protective effect of sodium selenite (SS) on DNA integrity, antioxidant/oxidant status, and histological changes on 4-nonylphenol (4-NP)-induced toxicity in liver and kidney tissues of rats. Twenty-four adult male Sprague Dawley rats were divided into 4 groups as control, SS, 4-NP, and SS+4-NP group. Control group was untreated. The SS group was supplemented with SS (0.5 mg/kg/day) and the 4-NP group was given 4-NP (125 mg/kg/day). The rats in the SS+4-NP group received SS followed by 4-NP 1 h later at the abovementioned doses. The treatments were administered by oral gavage for 48 days. DNA damage was analyzed by comet assay in lymphocytes. Oxidative stress parameters were measured, and histological evaluation was performed in liver and kidney tissues. Results showed that SS administration significantly decreased % Tail DNA and Mean Tail Moment in SS+4-NP group as compared with 4-NP group. Catalase activity in liver was significantly lower in 4-NP group only. SS treatment significantly increased the glutathione level and decreased high malondialdehyde level in tissues of the SS+4-NP group as compared with 4-NP group. Dilation of central vein, ballooning degeneration, vacuolar degeneration, and deterioration in the structure of remark cords in 4-NP-administered were alleviated in rats that received SS supplementation before administration of 4-NP. Moreover, glycogen intensity in hepatocytes and the wall of central vein increased in the SS+4-NP group. In addition, the SS supplementation in the SS+4-NP group decreased glomerular degeneration as well as the width of cavum glomeruli and congestion intensity in the kidney. These results indicate that SS may have a protective effect against 4-NP-induced hepato-nephrotoxicity in rats.
本研究旨在评估亚硒酸钠 (SS) 对 4-壬基酚 (4-NP) 诱导的大鼠肝、肾组织 DNA 完整性、抗氧化/氧化状态和组织学变化的保护作用。将 24 只成年雄性 Sprague Dawley 大鼠分为 4 组：对照组、SS 组、4-NP 组和 SS+4-NP 组。对照组未进行处理。SS 组给予 SS（0.5mg/kg/天），4-NP 组给予 4-NP（125mg/kg/天）。SS+4-NP 组的大鼠先给予 SS，1 小时后再给予上述剂量的 4-NP。通过口服灌胃的方式进行 48 天的治疗。通过彗星试验分析淋巴细胞中的 DNA 损伤。测量氧化应激参数，并对肝、肾组织进行组织学评价。结果表明，与 4-NP 组相比，SS+4-NP 组的 SS 给药显著降低了%尾 DNA 和平均尾矩。只有 4-NP 组的肝组织中过氧化氢酶活性显著降低。SS 处理可显著提高 SS+4-NP 组组织中的谷胱甘肽水平，降低丙二醛水平。与单独给予 4-NP 的大鼠相比，给予 SS 补充剂的大鼠肝组织中央静脉扩张、气球样变性、空泡变性和条索结构恶化得到缓解，中央静脉的糖原强度增加。此外，SS+4-NP 组的肾小球变性以及肾小球腔宽度和充血强度降低。这些结果表明，SS 可能对大鼠的 4-NP 诱导的肝-肾毒性具有保护作用。
