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运动员血液兴奋剂——通过流式细胞荧光术检测异体输血

Blood doping in athletes--detection of allogeneic blood transfusions by flow cytofluorometry.

作者信息

Arndt Patricia A, Kumpel Belinda M

机构信息

American Red Cross Blood Services, Southern California Region, Pomona, California 91768, USA.

出版信息

Am J Hematol. 2008 Aug;83(8):657-67. doi: 10.1002/ajh.21196.

DOI:10.1002/ajh.21196
PMID:18508326
Abstract

Athletes may undergo blood transfusion to increase their red cell mass and the oxygen carrying capacity of their blood in order to confer a competitive advantage. Allogeneic transfusions are normally mismatched at one or more minor blood group antigens. The most sensitive and accurate method known to detect this form of blood doping is flow cytometry. Low percentages of antigen-positive and antigen-negative red blood cells (RBCs) can be quantitated using suitable specific alloantibodies and careful analysis. By testing blood samples taken at various times, a reduction in the percentage of a minor population of RBCs will indicate transfusion has occurred.

摘要

运动员可能会接受输血以增加其红细胞量和血液的携氧能力,从而获得竞争优势。异体输血通常在一个或多个次要血型抗原上不匹配。已知检测这种血液兴奋剂形式最灵敏、最准确的方法是流式细胞术。使用合适的特异性同种抗体并进行仔细分析,可以对低比例的抗原阳性和抗原阴性红细胞(RBC)进行定量。通过检测在不同时间采集的血样,少数RBC群体百分比的降低将表明发生了输血。

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引用本文的文献

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Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.通过流式细胞荧光术检测运动兴奋剂中的同源输血:最新技术及降低假阴性结果风险的新方法
Front Sports Act Living. 2022 Feb 10;4:808449. doi: 10.3389/fspor.2022.808449. eCollection 2022.
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Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion.比较同种异体输血后早产儿同时进行的多种红细胞体积测定方法。
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Red blood cell populations and membrane levels of peroxiredoxin 2 as candidate biomarkers to reveal blood doping.
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Blood Transfus. 2012 May;10 Suppl 2(Suppl 2):s71-7. doi: 10.2450/2012.011S.
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ABO chimerism determined by real-time polymerase chain reaction analysis after ABO-incompatible haematopoietic stem cell transplantation.实时聚合酶链反应分析在 ABO 不相容造血干细胞移植后确定 ABO 嵌合体。
Blood Transfus. 2013 Jan;11(1):43-52. doi: 10.2450/2012.0013-12. Epub 2012 Jul 4.
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Red blood cell volume can be independently determined in vitro using sheep and human red blood cells labeled at different densities of biotin.可使用以不同生物素密度标记的绵羊和人类红细胞,在体外独立测定红细胞体积。
Transfusion. 2009 Jun;49(6):1178-85. doi: 10.1111/j.1537-2995.2009.02095.x. Epub 2009 Feb 10.