一种基于介孔二氧化硅纳米球的DNA转运体，其介孔表面由聚阳离子聚（烯丙胺盐酸盐）涂层介导。
A DNA transporter based on mesoporous silica nanospheres mediated with polycation poly(allylamine hydrochloride) coating on mesopore surface.
作者信息
Qin Fei, Zhou Yuchuan, Shi Jianlin, Zhang Yonglian
机构信息
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China.
出版信息
J Biomed Mater Res A. 2009 Aug;90(2):333-8. doi: 10.1002/jbm.a.31923.
Mesoporous silica nanospheres (MPNSs) were synthesized by the sol-gel method and were investigated by X-ray diffraction, nitrogen sorption, and transmission electron microscopy. The analyses showed that the MPNSs were highly dispersed, with a diameter range of 50-250 nm. Further, the polycation poly(allylamine hydrochloride) was coated on silica MPNSs to form a positively charged surface and to facilitate the loading of DNA molecules. The composite was successfully used as a intercellular carrier of DNA molecules, and this was confirmed by agarose gel electrophoresis and confocal laser scanning microscopy. Evidently, this composite has potential to be used as a carrier for a DNA delivery system.
采用溶胶-凝胶法合成了介孔二氧化硅纳米球（MPNSs），并通过X射线衍射、氮吸附和透射电子显微镜对其进行了研究。分析表明，MPNSs高度分散，直径范围为50-250nm。此外，将聚阳离子聚（烯丙胺盐酸盐）包覆在二氧化硅MPNSs上，以形成带正电的表面并促进DNA分子的负载。通过琼脂糖凝胶电泳和共聚焦激光扫描显微镜证实，该复合材料成功地用作DNA分子的细胞间载体。显然，这种复合材料有潜力用作DNA递送系统的载体。
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