Qin Fei, Zhou Yuchuan, Shi Jianlin, Zhang Yonglian
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China.
J Biomed Mater Res A. 2009 Aug;90(2):333-8. doi: 10.1002/jbm.a.31923.
Mesoporous silica nanospheres (MPNSs) were synthesized by the sol-gel method and were investigated by X-ray diffraction, nitrogen sorption, and transmission electron microscopy. The analyses showed that the MPNSs were highly dispersed, with a diameter range of 50-250 nm. Further, the polycation poly(allylamine hydrochloride) was coated on silica MPNSs to form a positively charged surface and to facilitate the loading of DNA molecules. The composite was successfully used as a intercellular carrier of DNA molecules, and this was confirmed by agarose gel electrophoresis and confocal laser scanning microscopy. Evidently, this composite has potential to be used as a carrier for a DNA delivery system.
采用溶胶-凝胶法合成了介孔二氧化硅纳米球（MPNSs），并通过X射线衍射、氮吸附和透射电子显微镜对其进行了研究。分析表明，MPNSs高度分散，直径范围为50-250nm。此外，将聚阳离子聚（烯丙胺盐酸盐）包覆在二氧化硅MPNSs上，以形成带正电的表面并促进DNA分子的负载。通过琼脂糖凝胶电泳和共聚焦激光扫描显微镜证实，该复合材料成功地用作DNA分子的细胞间载体。显然，这种复合材料有潜力用作DNA递送系统的载体。
