Lee Wilson, Low Bernard K M, Samaranayake Lakshman P, Hagg Urban
Orthodontics, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR.
Front Biosci. 2008 May 1;13:3814-24. doi: 10.2741/2970.
Candida is a common oral, opportunistic pathogen. The aim of this longitudinal study was to analyze the genotypes of sequential isolates of Candida albicans in a cohort of healthy consistent candidal carriers ('carriers') during fixed orthodontic appliance therapy. 11 of 97 subjects were 'carriers'. Candida isolates from baseline samples (T0) and sequential visits after insertion (T1 to T10) were speciated and RAPD fingerprint patterns of 101 sequential Candida albicans isolates were analyzed with PCR and gel electrophoresis. A similarity coefficient (SAB) for each pair of strains was calculated and clusters of similar strains grouped using Dendrogram analysis of the RAPD gel profiles. The composite dendrogram of all isolates indicated that the Candida populations in this cohort are genotypically dissimilar although collected from the same geographic locale and, from a similar healthy, age group. Genotypes of a majority of the cohort (6 of 11 consistent carriers) show minor evolutionary variations in profile and genotypic 'shuffling', implying this may be a survival mechanism of this common, human, opportunist pathogen residing in a hostile oral environment.
念珠菌是一种常见的口腔机会致病菌。这项纵向研究的目的是分析在固定正畸矫治器治疗期间，一组健康的持续性念珠菌携带者（“携带者”）中白色念珠菌连续分离株的基因型。97名受试者中有11名是“携带者”。对基线样本（T0）以及矫治器插入后的连续访视样本（T1至T10）中的念珠菌分离株进行菌种鉴定，并通过聚合酶链反应（PCR）和凝胶电泳分析101株连续白色念珠菌分离株的随机扩增多态性DNA（RAPD）指纹图谱。计算每对菌株的相似系数（SAB），并使用RAPD凝胶图谱的树形图分析对相似菌株进行聚类。所有分离株的复合树形图表明，尽管该队列中的念珠菌群体来自相同的地理位置且年龄组相似且健康状况相近，但它们在基因型上存在差异。大多数队列（11名持续性携带者中的6名）的基因型在图谱和基因型“洗牌”方面显示出微小的进化变异，这意味着这可能是这种常见的人类机会致病菌在恶劣口腔环境中的一种生存机制。
