Gyanchandani A, Khan Z K, Farooqui N, Goswami M, Ranade S A
Medical Mycology Division, Central Drug Research Institute, Lucknow, India.
Biochem Mol Biol Int. 1998 Jan;44(1):19-27. doi: 10.1080/15216549800201022.
The opportunistic imperfect fungus Candida albicans causing life-threatening infections in immunocompromised patients (ICP), especially in HIV-positive cases, is recognized to be one of the most important nosocomial pathogens in the recent decades. The extent of strain-to-strain variation within a species and its relationship to the ability of the organism to colonize or invade a specific group of patients or even a body site is, however, not well known. We have analysed 19 strains of C. albicans recovered from ICP at different locales and times, employing the RAPD technique. No two strains generated identical RAPD profiles with any of the 21 primers tested. Further, the UPGMA clustering of the strains seemingly reflected a certain relationship or nonrandomness in the infection of the patients with the strain of C. albicans vis-a-vis the immunocompromised status due to underlying disease such as diabetes, cancer, asthma and meningitis. These results may have a profound impact on the management of candidiasis, especially in the ICP.
机会性不完全真菌白色念珠菌在免疫功能低下患者（ICP）中引发危及生命的感染，尤其是在HIV阳性病例中，近几十年来它被公认为是最重要的医院病原体之一。然而，一个物种内菌株间变异的程度及其与生物体在特定患者群体甚至身体部位定植或侵袭能力的关系尚不清楚。我们采用RAPD技术分析了从不同地点和时间的ICP中分离出的19株白色念珠菌。在所测试的21种引物中，没有两个菌株产生相同的RAPD图谱。此外，菌株的UPGMA聚类似乎反映了白色念珠菌菌株感染患者与因糖尿病、癌症、哮喘和脑膜炎等基础疾病导致的免疫功能低下状态之间的某种关系或非随机性。这些结果可能对念珠菌病的管理产生深远影响，尤其是在ICP中。
