Hoelter Sabine M, Dalke Claudia, Kallnik Magdalena, Becker Lore, Horsch Marion, Schrewe Anja, Favor Jack, Klopstock Thomas, Beckers Johannes, Ivandic Boris, Gailus-Durner Valérie, Fuchs Helmut, Hrabé de Angelis Martin, Graw Jochen, Wurst Wolfgang
Institute of Developmental Genetics, Helmholtz Center Munich-German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany.
Front Biosci. 2008 May 1;13:5810-23. doi: 10.2741/3118.
It is unclear what role vision plays in guiding mouse behaviour, since the mouse eye is of comparably low optical quality, and mice are considered to rely primarily on other senses. All C3H substrains are homozygous for the Pde6b(rd1) mutation and get blind by weaning age. To study the impact of the Pde6b(rd1) mutation on mouse behaviour and physiology, sighted C3H (C3H.Pde6b+) and normal C3H/HeH mice were phenotyped for different aspects. We confirmed retinal degeneration 1 in C3H/HeH mice, and the presence of a morphologically normal retina as well as visual ability in C3H.Pde6b+ mice. However, C3H.Pde6b+ mice showed an abnormal retinal function in the electroretinogram response, indicating that their vision was not normal as expected. C3H.Pde6b+ mice showed reduced latencies for several behaviours without any further alterations in these behaviours in comparison to C3H/HeH mice, suggesting that visual ability, although impaired, enables earlier usage of the behavioural repertoire in a novel environment, but does not lead to increased activity levels. These results emphasize the importance of comprehensive behavioural and physiological phenotyping.
目前尚不清楚视觉在引导小鼠行为中所起的作用，因为小鼠眼睛的光学质量相对较低，且人们认为小鼠主要依赖其他感官。所有C3H亚系对于Pde6b(rd1)突变都是纯合的，并且在断奶时会失明。为了研究Pde6b(rd1)突变对小鼠行为和生理的影响，对有视力的C3H（C3H.Pde6b+）和正常的C3H/HeH小鼠在不同方面进行了表型分析。我们证实了C3H/HeH小鼠存在视网膜变性1，以及C3H.Pde6b+小鼠存在形态正常的视网膜和视觉能力。然而，C3H.Pde6b+小鼠在视网膜电图反应中显示出异常的视网膜功能，这表明它们的视力并不像预期的那样正常。与C3H/HeH小鼠相比，C3H.Pde6b+小鼠在几种行为上的潜伏期缩短，且这些行为没有任何进一步的改变，这表明视觉能力虽然受损，但能使小鼠在新环境中更早地运用行为模式，但不会导致活动水平增加。这些结果强调了全面的行为和生理表型分析的重要性。
