Omori Atsuko, Takahashi Kenji, Hazawa Masaharu, Misaki Naoko, Ohba Hisateru, Manabe Mami, Sato Haruo, Kudo Kaori, Takahashi Tsuneo A, Kashiwakura Ikuo
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
Tohoku J Exp Med. 2008 May;215(1):23-32. doi: 10.1620/tjem.215.23.
Placental/umbilical cord blood (CB) contains nucleated cells and hematopoietic stem/progenitor cells (CD34(+) cells). However it is difficult to predict the number of nucleated/CD34(+) cells in each CB before cell processing. Despite many previous studies from institutes affiliated with CB banks in metropolitan areas, little information is available regarding the characteristics of CB units from other medical facilities. The purpose of the present study was to analyze the maternal/neonatal factors on the yield of cells in CB units. A total of 176 CB units were obtained from single-birth and normal vaginal deliveries. Mononuclear low-density (LD) cells were separated using Ficoll-Paque within 24 hrs after CB collection and then processed for the purification of CD34(+) cells. A multiple linear regression analysis was performed to assess the correlations between the yield of cells and maternal/neonatal factors including maternal age, gravid status, duration of labor, gestational age, neonatal height and weight, cord length, and meconium in the amniotic fluid. The total LD cells per CB unit had a weak positive correlation with the maternal age of primigravidae. The total LD cells per CB unit from the primigravidae aged > or = 25 were significantly higher than those from the primigravidae aged < or = 24. The total CD34(+) cells per CB unit from the 1-gravidae were significantly higher than those from the 2-gravidae and 3-gravidae, respectively among all donors. These results indicate that the CB units from the primigravidae aged > or = 25 are more likely to contain higher yield of LD/CD34(+) cells.
胎盘/脐带血（CB）含有有核细胞和造血干/祖细胞（CD34(+)细胞）。然而，在细胞处理前很难预测每份CB中有核/CD34(+)细胞的数量。尽管之前有许多来自大都市地区CB库附属机构的研究，但关于其他医疗机构CB样本特征的信息却很少。本研究的目的是分析影响CB样本中细胞产量的母体/新生儿因素。共采集了176份单胎顺产的CB样本。CB采集后24小时内，使用Ficoll-Paque分离单核低密度（LD）细胞，然后进行CD34(+)细胞的纯化处理。进行多元线性回归分析，以评估细胞产量与母体/新生儿因素之间的相关性，这些因素包括产妇年龄、妊娠状态、产程、孕周、新生儿身高和体重、脐带长度以及羊水中的胎粪。每份CB样本中的总LD细胞数与初产妇的年龄呈弱正相关。年龄≥25岁的初产妇每份CB样本中的总LD细胞数显著高于年龄≤24岁的初产妇。在所有捐赠者中，初产妇每份CB样本中的总CD34(+)细胞数分别显著高于经产妇和三产妇。这些结果表明，年龄≥25岁的初产妇的CB样本更有可能含有较高产量的LD/CD34(+)细胞。
