He Jing, Yang Yue-Ming, Liu Ling, Wang Dong
Graduate School of Dalian Medical University, Dalian, Liaoning 116000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2021 Jan;23(1):43-48. doi: 10.7499/j.issn.1008-8830.2008172.
To study the association of different maternal and infant factors with the number of total nucleated cells and CD34 hematopoietic stem/progenitor cells in umbilical cord blood, and to provide a reference for reasonable selection of umbilical cord blood in the cord blood bank.
A prospective study was performed for the umbilical cord blood samples of 130 neonates who were born in Dalian Women and Children's Medical Center from June 2019 to January 2020, with a male/female ratio of 1:1. Related perinatal information was collected, including maternal age and blood type, presence or absence of gestational diabetes or gestational hypertension, pregnancy method, mode of delivery, singleton pregnancy/twin pregnancy, body weight and sex of neonates, Apgar score after birth, and the conditions of placenta, amniotic fluid, and umbilical cord.
The neonates were grouped according to maternal blood type, gestational diabetes, gestational hypertension, pregnancy method, mode of delivery, singleton pregnancy/ twin pregnancy, sex of neonates, Apgar score after birth, placental morphology, meconium staining of amniotic fluid, and umbilical cord around the neck. The comparison between groups showed no significant differences in the numbers of total nucleated cells and CD34 cells in umbilical cord blood ( > 0.05). Maternal age and neonatal body weight were not correlated with the number of total nucleated cells in umbilical cord blood ( > 0.05), and neonatal body weight was not correlated with the number of CD34 cells ( > 0.05), while maternal age was positively correlated with the number of CD34 cells ( < 0.05).
The number of CD34 cells in umbilical cord blood increases with the increase in maternal age, and therefore, umbilical cord blood in the cord blood bank may be selected based on maternal age.
研究不同母婴因素与脐带血中总核细胞及CD34造血干/祖细胞数量的相关性，为脐带血库合理选择脐带血提供参考。
对2019年6月至2020年1月在大连市妇女儿童医疗中心出生的130例新生儿的脐带血样本进行前瞻性研究，男女比例为1:1。收集相关围产期信息，包括母亲年龄、血型、是否患有妊娠期糖尿病或妊娠期高血压、妊娠方式、分娩方式、单胎/双胎妊娠、新生儿体重和性别、出生后阿氏评分以及胎盘、羊水和脐带情况。
根据母亲血型、妊娠期糖尿病、妊娠期高血压、妊娠方式、分娩方式、单胎/双胎妊娠、新生儿性别、出生后阿氏评分、胎盘形态、羊水胎粪污染及脐带绕颈情况对新生儿进行分组。组间比较显示，脐带血中总核细胞和CD34细胞数量差异无统计学意义（>0.05）。母亲年龄和新生儿体重与脐带血中总核细胞数量无相关性（>0.05），新生儿体重与CD34细胞数量无相关性（>0.05），而母亲年龄与CD34细胞数量呈正相关（<0.05）。
脐带血中CD34细胞数量随母亲年龄增加而增多，因此，脐带血库可根据母亲年龄选择脐带血。
