Yao Ke, Wu Wei, Wang KaiJun, Ni Shuang, Ye PanPan, Yu YiBo, Ye Juan, Sun LiXia
Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University, Hangzhou,China.
Mol Vis. 2008 May 19;14:964-9.
The goal of this study was to investigate whether superposing of electromagnetic noise could block or attenuate DNA damage and intracellular reactive oxygen species (ROS) increase of cultured human lens epithelial cells (HLECs) induced by acute exposure to 1.8 GHz radiofrequency field (RF) of the Global System for Mobile Communications (GSM).
An sXc-1800 RF exposure system was used to produce a GSM signal at 1.8 GHz (217 Hz amplitude-modulated) with the specific absorption rate (SAR) of 1, 2, 3, and 4 W/kg. After 2 h of intermittent exposure, the ROS level was assessed by the fluorescent probe, 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). DNA damage to HLECs was examined by alkaline comet assay and the phosphorylated form of histone variant H2AX (gammaH2AX) foci formation assay.
After exposure to 1.8 GHz RF for 2 h, HLECs exhibited significant intracellular ROS increase in the 2, 3, and 4 W/kg groups. RF radiation at the SAR of 3 W/kg and 4 W/kg could induce significant DNA damage, examined by alkaline comet assay, which was used to detect mainly single strand breaks (SSBs), while no statistical difference in double strand breaks (DSBs), evaluated by gammaH2AX foci, was found between RF exposure (SAR: 3 and 4 W/kg) and sham exposure groups. When RF was superposed with 2 muT electromagnetic noise could block RF-induced ROS increase and DNA damage.
DNA damage induced by 1.8 GHz radiofrequency field for 2 h, which was mainly SSBs, may be associated with the increased ROS production. Electromagnetic noise could block RF-induced ROS formation and DNA damage.
本研究旨在探究叠加电磁噪声是否能够阻断或减轻急性暴露于全球移动通信系统（GSM）1.8 GHz射频场（RF）所诱导的培养人晶状体上皮细胞（HLECs）的DNA损伤及细胞内活性氧（ROS）增加。
使用sXc - 1800 RF暴露系统产生1.8 GHz（217 Hz调幅）的GSM信号，比吸收率（SAR）分别为1、2、3和4 W/kg。间歇暴露2小时后，通过荧光探针2',7'-二氯二氢荧光素二乙酸酯（DCFH - DA）评估ROS水平。通过碱性彗星试验和组蛋白变体H2AX磷酸化形式（γH2AX）焦点形成试验检测HLECs的DNA损伤。
暴露于1.8 GHz RF 2小时后，2、3和4 W/kg组的HLECs细胞内ROS显著增加。通过碱性彗星试验检测发现，3 W/kg和4 W/kg SAR的RF辐射可诱导显著的DNA损伤，该试验主要用于检测单链断裂（SSBs），而通过γH2AX焦点评估的双链断裂（DSBs）在RF暴露组（SAR：3和4 W/kg）与假暴露组之间未发现统计学差异。当RF与2 μT电磁噪声叠加时，可阻断RF诱导的ROS增加和DNA损伤。
1.8 GHz射频场暴露2小时所诱导的DNA损伤主要为SSBs，可能与ROS生成增加有关。电磁噪声可阻断RF诱导的ROS形成和DNA损伤。
