Randhawa Swaran S, Dua K, Randhawa C S, Randhawa S S, Munshi S K
Department of Clinical Veterinary Medicine, Ethics & Jurisprudence, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Vet Res Commun. 2008 Dec;32(8):599-608. doi: 10.1007/s11259-008-9060-z. Epub 2008 May 29.
The effect of biotin supplementation on various foot lesions and hoof ceramide composition of toe (wall) and sole portions of hooves was studied in crossbred dairy cattle. Biotin supplementation was done for five months in 14 cattle at a farm and the other 14 animals kept as control. A significant decline was observed in heel erosions and sole avulsions along with total disappearance of white line fissures and double soles in the biotin supplemented cattle resulting in decrease in the overall disease score. Thin layer chromatographs of the hoof lipids revealed 11 types of ceramides in sole lipids and 6 types of ceramides in toe (wall) lipids. The ceramides were typed and identified according to their Rf values. A qualitative increase in the density of thin layer chromatographs of sole lipids was observed in biotin supplemented cattle whereas a non-significant difference in density of thin layer chromatographs of toe lipids was observed after supplementation of biotin.
在杂交奶牛中研究了补充生物素对各种足部病变以及蹄趾（壁）和蹄底部分的蹄神经酰胺组成的影响。在一个农场，对14头牛进行了为期五个月的生物素补充，另外14头牛作为对照。在补充生物素的牛中，观察到足跟糜烂和蹄底撕裂显著减少，同时白线裂缝和双蹄底完全消失，导致总体疾病评分降低。蹄脂的薄层色谱显示，蹄底脂质中有11种神经酰胺，蹄趾（壁）脂质中有6种神经酰胺。根据它们的比移值对神经酰胺进行分类和鉴定。在补充生物素的牛中，观察到蹄底脂质薄层色谱密度有定性增加，而补充生物素后，蹄趾脂质薄层色谱密度无显著差异。
