Stepien H, Lyson K, Stanisz A M, Pawlikowski M
Department of Experimental Endocrinology, University School of Medicine, Lodz, Poland.
Int J Immunopharmacol. 1991;13(1):51-6. doi: 10.1016/0192-0561(91)90025-3.
Nerve growth factor (NGF), a trophic neuropeptide, is known to stimulate development, and to be important in the maintenance and survival of sympathetic and sensory neurons. Considering the presence of specific receptors on the surface of spleen cells, the effect of 2.5s nerve growth factor on 3H-thymidine uptake, cAMP and cGMP accumulation in mouse spleen lymphocytes has been studied. It was found that NGF added in vitro at the concentrations between 4 x 10(-7) and 4 x 10(-8) M significantly inhibited the incorporation of 3H-thymidine into lymphocytes DNA and increased cAMP levels in a dose-dependent manner but had no effect on cGMP levels. The maximal stimulation of cAMP synthesis occurred between 5 and 30 min after the NGF addition to the culture medium. When NGF was administered in vivo a significant dose-dependent inhibition of the lymphocytes proliferation was observed. These results indicate that an early increase of cAMP concentration is responsible for the antiproliferative action of NGF on mouse spleen lymphocytes and suggest that NGF could play an important role in the regulation of immune system function.
神经生长因子（NGF）是一种营养性神经肽，已知其可刺激发育，并在交感神经元和感觉神经元的维持与存活中发挥重要作用。鉴于脾细胞表面存在特异性受体，研究了2.5s神经生长因子对小鼠脾淋巴细胞摄取³H-胸腺嘧啶核苷、环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）积累的影响。结果发现，体外添加浓度在4×10⁻⁷至4×10⁻⁸M之间的NGF可显著抑制³H-胸腺嘧啶核苷掺入淋巴细胞DNA，并以剂量依赖方式提高cAMP水平，但对cGMP水平无影响。在向培养基中添加NGF后5至30分钟内，cAMP合成出现最大刺激。当体内给予NGF时，观察到淋巴细胞增殖受到显著的剂量依赖性抑制。这些结果表明，cAMP浓度的早期升高是NGF对小鼠脾淋巴细胞抗增殖作用的原因，并提示NGF可能在免疫系统功能调节中发挥重要作用。
