Weinstein Y, Segal S, Melmon K L
J Immunol. 1975 Jul;115(1):112-7.
8-Br-cyclic GMP has been found to be a specific B cell mitogen; it triggers athymic nude mice spleen cells and "B mice" spleen cells, nylon adherent, anti-theta and complement-treated cells to proliferate. It does not stimulate thymocytes or purified T cells. The kinetics of the response to Br-cyclic GMP and LPS are almost identical. The mitogenic effect of LPS and Br-cyclic GMP is additive when the two mitogens are given together to cells. Spleen cells (C3H/HeJ strain) that did not respond to LPS were triggered by Br-cyclic GMP to make DNA. In order to achieve maximal stimulation by Br-cyclic GMP, the drug had to be in contact with the cells for more than 24 hr. Br-cyclic GMP was found to be mitogenic for spleen cells from five different mouse strains, but not for human leukocytes. DB-cyclic AMP was found to inhibit the DNA synthesis of T lymphocytes after they interacted with Con A; DB-cyclic AMP had no effect on the ability of the B lymphocytes to be transformed by LPS. The differential effects of cyclic nucleotides on B vs. T lymphocytes are discussed.
已发现8-溴环鸟苷酸是一种特异性B细胞促有丝分裂原；它能触发无胸腺裸鼠脾细胞以及“B小鼠”脾细胞、尼龙黏附细胞、抗θ及补体处理细胞增殖。它不刺激胸腺细胞或纯化的T细胞。对溴环鸟苷酸和脂多糖的反应动力学几乎相同。当将这两种促有丝分裂原一起给予细胞时，脂多糖和溴环鸟苷酸的促有丝分裂作用是相加的。对脂多糖无反应的脾细胞（C3H/HeJ品系）被溴环鸟苷酸触发合成DNA。为了通过溴环鸟苷酸实现最大刺激，该药物必须与细胞接触超过24小时。已发现溴环鸟苷酸对五种不同小鼠品系的脾细胞有促有丝分裂作用，但对人白细胞无此作用。已发现二丁酰环磷腺苷在T淋巴细胞与刀豆球蛋白A相互作用后抑制其DNA合成；二丁酰环磷腺苷对B淋巴细胞被脂多糖转化的能力无影响。文中讨论了环核苷酸对B淋巴细胞和T淋巴细胞的不同作用。
