新生大鼠嗅觉神经元的原代培养。
Primary culture of neonatal rat olfactory neurons.
作者信息
Ronnett G V, Hester L D, Snyder S H
机构信息
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
出版信息
J Neurosci. 1991 May;11(5):1243-55. doi: 10.1523/JNEUROSCI.11-05-01243.1991.
Abstract
We have prepared primary cultures of purified neonatal rat olfactory neurons. Dissociated olfactory epithelial cells are maintained in modified Eagle's medium with D-valine, cytosine arabinoside, and NGF. NGF is required for neuronal survival. Immunohistochemical staining is positive for the neuronal markers vimentin, olfactory marker protein, and neuron-specific enolase, but negative for the glial markers, glial fibrillary acidic protein, and S-100 protein. Physiologic concentrations of odorants stimulate cAMP accumulation in the cells. Because of their morphology, biochemical composition, and responsiveness to odorants, these cells should enhance olfactory investigations.
摘要
我们已经制备了纯化的新生大鼠嗅觉神经元的原代培养物。解离的嗅觉上皮细胞在含有D-缬氨酸、阿糖胞苷和神经生长因子（NGF）的改良伊格尔培养基中培养。神经生长因子是神经元存活所必需的。免疫组织化学染色对神经元标志物波形蛋白、嗅觉标志物蛋白和神经元特异性烯醇化酶呈阳性，但对神经胶质标志物胶质纤维酸性蛋白和S-100蛋白呈阴性。生理浓度的气味剂可刺激细胞内cAMP的积累。由于它们的形态、生化组成以及对气味剂的反应性，这些细胞应该会加强嗅觉研究。
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