Cameron H A, Woolley C S, McEwen B S, Gould E
Laboratory of Neuroendocrinology, Rockefeller University, New York, NY 10021.
Neuroscience. 1993 Sep;56(2):337-44. doi: 10.1016/0306-4522(93)90335-d.
In order to determine whether newly born cells in the dentate gyrus of the adult rat express the neuronal marker, neuron-specific enolase, or the glial marker, glial fibrillary acidic protein, we performed combined immunohistochemistry and autoradiography on brains from adult rats perfused at various times ranging from 1 h to four weeks following [3H]thymidine administration. Light-microscopic examination revealed a negligible number of [3H]thymidine-labeled cells showing neuron-specific enolase immunoreactivity during mitosis. However, by two weeks after [3H]thymidine administration, a significant increase in the density of [3H]thymidine-labeled neuron-specific enolase-immunoreactive cells was detected. Three weeks following [3H]thymidine injection the majority of [3H]thymidine-labeled cells (> 70%) were immunoreactive for the neuronal marker. At the four-week time-point, [3H]thymidine-labeled neuron-specific enolase-immunoreactive cells were indistinguishable from neighboring granule cells. In contrast, glial fibrillary acidic protein immunoreactivity was observed in a small but significant number of [3H]thymidine cells at the 1-h time-point and the proportion of labeled cells that were immunoreactive for this cell marker did not increase with time. [3H]Thymidine-labeled cells that were immunoreactive for glial fibrillary acidic protein typically showed morphologic characteristics of radial glia at all time-points. At the 1-h time-point, the majority of [3H]thymidine-labeled cells were observed in the hilus (> 60%) with the remainder being located in the granule cell layer. However, with a four-week survival-time most [3H]thymidine-labeled cells (> 85%) were located in the granule cell layer. The majority of newly born cells in the adult dentate gyrus differentiate into neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
为了确定成年大鼠齿状回中新生细胞是否表达神经元标志物神经元特异性烯醇化酶或胶质标志物胶质纤维酸性蛋白，我们对成年大鼠在给予[3H]胸腺嘧啶核苷后1小时至四周内不同时间灌注的大脑进行了免疫组织化学和放射自显影联合实验。光学显微镜检查显示，在有丝分裂期间，显示神经元特异性烯醇化酶免疫反应性的[3H]胸腺嘧啶核苷标记细胞数量可忽略不计。然而，在给予[3H]胸腺嘧啶核苷两周后，检测到[3H]胸腺嘧啶核苷标记的神经元特异性烯醇化酶免疫反应性细胞密度显著增加。在注射[3H]胸腺嘧啶核苷三周后，大多数[3H]胸腺嘧啶核苷标记细胞（>70%）对神经元标志物呈免疫反应性。在四周时间点，[3H]胸腺嘧啶核苷标记的神经元特异性烯醇化酶免疫反应性细胞与相邻的颗粒细胞无明显差异。相比之下，在1小时时间点，在少量但显著数量的[3H]胸腺嘧啶核苷细胞中观察到胶质纤维酸性蛋白免疫反应性，并且对该细胞标志物呈免疫反应性的标记细胞比例并未随时间增加。对胶质纤维酸性蛋白呈免疫反应性的[3H]胸腺嘧啶核苷标记细胞在所有时间点通常都显示出放射状胶质细胞的形态特征。在1小时时间点，大多数[3H]胸腺嘧啶核苷标记细胞（>60%）出现在门区，其余位于颗粒细胞层。然而，在四周存活期时，大多数[3H]胸腺嘧啶核苷标记细胞（>85%）位于颗粒细胞层。成年齿状回中的大多数新生细胞分化为神经元。（摘要截短至250字）
