Meermann Björn, Bartel Marc, Scheffer Andy, Trümpler Stefan, Karst Uwe
Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany.
Electrophoresis. 2008 Jun;29(12):2731-7. doi: 10.1002/elps.200700902.
CE was coupled to inductively coupled plasma MS (ICP-MS) and ESI-MS to identify and quantify the arsenic species arsenobetaine (AsB), arsenite (As(III)), arsenate (As(V)), and dimethylarsinic acid (DMA). A GC-flame ionization detector (FID)-based German standard method and ICP-MS were used for validation of the data obtained for arsenobetaine and total arsenic, respectively. LODs obtained with the CE-ESI-TOF-MS method were 1.0x10(-7) M for AsB, 5.0x10(-7) M for DMA, and 1.0x10(-6) M for As(III) and As(V). For the CE-ICP-MS method, LODs were 8.5x10(-8) M for AsB, 9.5x10(-8) M for DMA, 9.3x10(-8) M for As(III), and 6.2x10(-8) M for As(V). While CE-ICP-MS provided high sensitivity and better reproducibility for quantitative measurements, CE-ESI-MS with a TOF mass analyzer proved to be valuable for species identification. With this setup, fish samples were prepared and analyzed and the obtained data were successfully validated with the independent methods.
毛细管电泳（CE）与电感耦合等离子体质谱（ICP-MS）和电喷雾电离质谱（ESI-MS）联用，以鉴定和定量砷甜菜碱（AsB）、亚砷酸盐（As(III)）、砷酸盐（As(V)）和二甲基砷酸（DMA）等砷形态。基于气相色谱-火焰离子化检测器（FID）的德国标准方法和ICP-MS分别用于验证砷甜菜碱和总砷所获得的数据。CE-ESI-TOF-MS方法获得的检测限，对于AsB为1.0×10⁻⁷ M，对于DMA为5.0×10⁻⁷ M，对于As(III)和As(V)为1.0×10⁻⁶ M。对于CE-ICP-MS方法，检测限对于AsB为8.5×10⁻⁸ M，对于DMA为9.5×10⁻⁸ M，对于As(III)为9.3×10⁻⁸ M，对于As(V)为6.2×10⁻⁸ M。虽然CE-ICP-MS为定量测量提供了高灵敏度和更好的重现性，但带有飞行时间质量分析器的CE-ESI-MS被证明对形态鉴定很有价值。通过这种设置，制备并分析了鱼类样品，所获得的数据用独立方法成功进行了验证。
