Michalke B, Schramel P
GSF-National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany.
Electrophoresis. 1998 Sep;19(12):2220-5. doi: 10.1002/elps.1150191229.
A method is presented to separate and detect six arsenic species by capillary electrophoresis (CE) interfaced to inductively coupled plasma mass spectrometry (ICP-MS). CE was used as a highly resolving separation system, whereas ICP-MS served as an element selective detector providing low detection limits. The special mode of operation included sample stacking and a differentiation of separation and detection. This provided separation and detection of six As species, uncharged and anionic, to be monitored within a single run. Detection limits were calculated according to IUPAC recommendation at 15 microg As/L for As (III), dimethyl arsinic acid (DA), monomethyl arsonic acid (MA) and As (V), or 65 microg As/L for arsenobetaine (AsB) and arsenocholine (AsC). Investigations were focused on possibly occurring interferences, e.g., ArCl+ interference at the monoisotope 75As. Finally, real samples from biomedical field (urine) and environmental field (sewage sludge) were analyzed.
本文介绍了一种通过与电感耦合等离子体质谱（ICP-MS）联用的毛细管电泳（CE）来分离和检测六种砷形态的方法。CE用作高分辨率分离系统，而ICP-MS则作为提供低检测限的元素选择性检测器。特殊的操作模式包括样品堆积以及分离和检测的区分。这使得在一次运行中能够对六种砷形态（不带电荷的和阴离子的）进行分离和检测。根据国际纯粹与应用化学联合会（IUPAC）的建议计算出的检测限为：对于As（III）、二甲基砷酸（DA）、一甲基砷酸（MA）和As（V）为15μg As/L，对于砷甜菜碱（AsB）和砷胆碱（AsC）为65μg As/L。研究集中在可能出现的干扰，例如在单同位素75As处的ArCl+干扰。最后，对生物医学领域（尿液）和环境领域（污水污泥）的实际样品进行了分析。
