小鼠组织中气态信号分子硫化氢的产生。
Production of the gaseous signal molecule hydrogen sulfide in mouse tissues.
作者信息
Linden David R, Sha Lei, Mazzone Amelia, Stoltz Gary J, Bernard Cheryl E, Furne Julie K, Levitt Michael D, Farrugia Gianrico, Szurszewski Joseph H
机构信息
Enteric NeuroScience Program, and Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA.
出版信息
J Neurochem. 2008 Aug;106(4):1577-85. doi: 10.1111/j.1471-4159.2008.05502.x. Epub 2008 May 29.
Abstract
The gaseous molecule hydrogen sulfide (H(2)S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H(2)S. This production occurs through the activity of two enzymes, cystathionine-gamma-lyase and cystathionine-beta-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H(2)S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.
摘要
气态分子硫化氢（H₂S）已被认为是哺乳动物体内的一种内源性信号分子和神经调节剂。我们使用一种新开发的方法，首次在此报告了小鼠完整且有生命的脑和结肠组织生成并释放H₂S的能力。这种生成是通过两种酶，即胱硫醚-γ-裂解酶和胱硫醚-β-合酶的活性实现的。描述了这两种酶在肝脏、脑和结肠中的信使核糖核酸的定量表达及蛋白质定位。酶的表达水平在不同组织间存在差异，且分布也不同。对外源性施用的H₂S有反应的组织能够内源性生成这种气体，这一观察结果有力地支持了其作为内源性信号分子的作用。
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Neuroscience. 2008 Mar 3;152(1):89-96. doi: 10.1016/j.neuroscience.2007.11.052.
2
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Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):17977-82. doi: 10.1073/pnas.0705710104. Epub 2007 Oct 19.
3
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Eur J Pharmacol. 2007 Mar 15;559(1):65-8. doi: 10.1016/j.ejphar.2006.12.011. Epub 2007 Jan 17.
4
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