Hirata A, Kitaoka T, Ishigooka H, Ueno S
Department of Ophthalmology, Kyoto University Faculty of Medicine, Japan.
Acta Ophthalmol (Copenh). 1991 Feb;69(1):71-5. doi: 10.1111/j.1755-3768.1991.tb01995.x.
We have investigated the fine structural features of the transitional area between retina and optic nerve with special emphasis on the identification of Müller cells using the cytochemical method for detecting the localization of G6Pase activity, which is specific to the endoplasmic reticulum (ER) of Müller cells in the guinea pig retina. This cytochemical method enabled us to differentiate Müller cells from astrocytes or other cells easily and also allowed topographical, three dimensional analysis by high voltage electron microscopy because of the high contrast of the reaction products. Our study demonstrates that Müller cells are a major constitutional element of intermediary tissue of Kuhnt, which is considered to be the possible site of barrier between optic nerve and retina. These findings may indicate that Müller cells contribute to the barrier function in this region of the eye.
我们研究了视网膜与视神经之间过渡区域的精细结构特征，特别着重于使用细胞化学方法鉴定 Müller 细胞，该方法用于检测葡萄糖-6-磷酸酶（G6Pase）活性的定位，G6Pase 活性是豚鼠视网膜中 Müller 细胞内质网（ER）所特有的。这种细胞化学方法使我们能够轻松地将 Müller 细胞与星形胶质细胞或其他细胞区分开来，并且由于反应产物的高对比度，还可以通过高压电子显微镜进行局部的三维分析。我们的研究表明，Müller 细胞是 Kuhnt 中间组织的主要组成元素，Kuhnt 中间组织被认为可能是视神经与视网膜之间屏障的部位。这些发现可能表明 Müller 细胞在眼睛的该区域发挥屏障功能。
