奥昔布宁和N-去乙基奥昔布宁的对映体选择性分析及其在体外生物转化研究中的应用。
Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study.
作者信息
da Fonseca Patrícia, de Freitas Luis Alexandre Pedro, Pinto Luis Felipe Ribeiro, Pestana Cezar Rangel, Bonato Pierina Sueli
机构信息
Faculdade de Ciências Farmacâeuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 1;875(1):161-7. doi: 10.1016/j.jchromb.2008.05.023.
An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.
建立了一种采用液相微萃取（LPME）后接高效液相色谱（HPLC）分析的对映体选择性方法，用于测定大鼠肝微粒体组分中奥昔布宁（OXY）及其主要代谢物N-去乙基奥昔布宁（DEO）。通过多因素实验对LPME程序进行了优化。在最佳萃取条件下，（R）-OXY和（S）-OXY的平均回收率分别为61%和55%，（R）-DEO和（S）-DEO的平均回收率分别为70%和76%。该经过验证的方法用于大鼠肝微粒体组分的体外生物转化研究。结果表明奥昔布宁存在对映体选择性生物转化。
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