Denzel Andrea, Maus Ulrich A, Rodriguez Gomez Manuel, Moll Cordula, Niedermeier Marianne, Winter Christine, Maus Regina, Hollingshead Susan, Briles David E, Kunz-Schughart Leoni A, Talke Yvonne, Mack Matthias
Department of Internal Medicine II, University Hospital Regensburg, 93042 Regensburg, Germany.
Nat Immunol. 2008 Jul;9(7):733-42. doi: 10.1038/ni.1621. Epub 2008 May 30.
The cellular basis of immunological memory remains a controversial issue. Here we show that basophils bound large amounts of intact antigens on their surface and were the main source of interleukins 6 and 4 in the spleen and bone marrow after restimulation with a soluble antigen. Depletion of basophils resulted in a much lower humoral memory response and greater susceptibility of immunized mice to sepsis induced by Streptococcus pneumoniae. Adoptive transfer of antigen-reactive basophils significantly increased specific antibody production, and activated basophils, together with CD4(+) T cells, profoundly enhanced B cell proliferation and immunoglobulin production. These basophil-dependent effects on B cells required interleukins 6 and 4 and increased the capacity of CD4(+) T cells to provide B cell help. Thus, basophils are important contributors to humoral memory immune responses.
免疫记忆的细胞基础仍是一个有争议的问题。我们在此表明，嗜碱性粒细胞在其表面结合大量完整抗原，并且在用可溶性抗原再次刺激后，是脾脏和骨髓中白细胞介素6和4的主要来源。嗜碱性粒细胞的耗竭导致体液记忆反应显著降低，且免疫小鼠对肺炎链球菌诱导的败血症更易感。抗原反应性嗜碱性粒细胞的过继转移显著增加特异性抗体产生，并且活化的嗜碱性粒细胞与CD4(+) T细胞一起，深刻增强B细胞增殖和免疫球蛋白产生。这些嗜碱性粒细胞对B细胞的依赖性作用需要白细胞介素6和4，并增加CD4(+) T细胞提供B细胞辅助的能力。因此，嗜碱性粒细胞是体液记忆免疫反应的重要贡献者。
