Müller F, Laufer W, Pott U, Ciriacy M
Institut für Mikrobiologie, Universität Düsseldorf, Federal Republic of Germany.
Mol Gen Genet. 1991 Apr;226(1-2):145-53. doi: 10.1007/BF00273598.
Transposition of the yeast transposable element, Ty, has been shown to require a reverse transcription process. By analysing the extrachromosomal Ty-specific nucleic acid molecules associated with overproduced Ty virus-like particles (Ty-VLPs), we identified several reverse transcribed cDNA strands. Most of them resemble the characteristic intermediates of the reverse transcription process described for authentic retroviruses: a (-) strong-stop DNA strand covalently bound to an RNA primer, two elongated (-) strands with one or two long terminal repeat (LTR) sequences and a (+) strong-stop DNA. Surprisingly, complete (+) strands and full-length linear duplex Ty DNA could not be detected. The structural features of two additional (+) strands may indicate some differences between the mechanisms of (+) strand synthesis in Ty and other retrotransposons or retroviruses.
酵母转座元件Ty的转座已被证明需要逆转录过程。通过分析与过量产生的Ty病毒样颗粒（Ty-VLPs）相关的染色体外Ty特异性核酸分子，我们鉴定出了几条逆转录的cDNA链。其中大多数类似于针对正宗逆转录病毒所描述的逆转录过程的特征性中间体：一条与RNA引物共价结合的（-）强终止DNA链、两条带有一个或两个长末端重复（LTR）序列的延长（-）链以及一条（+）强终止DNA。令人惊讶的是，未检测到完整的（+）链和全长线性双链Ty DNA。另外两条（+）链的结构特征可能表明Ty与其他逆转座子或逆转录病毒在（+）链合成机制上存在一些差异。
