Tamm I, Cardinale I, Murphy J S
Cell Physiology and Virology Laboratory, Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4414-8. doi: 10.1073/pnas.88.10.4414.
Interleukin 6 (IL-6) has been shown to inhibit the proliferation, but increase the motility, of wild-type ZR-75-1 human ductal breast carcinoma cells, a line of cells that resemble closely the malignant cells cultured from the ascitic effusion. IL-6-treated cells lose their epithelial character, become stellate or fusiform in shape, and migrate away from neighbors. In the wild-type ZR-75-1 cells, IL-6 causes cell-cell separation in preformed colonies as well as postmitotically. We have now investigated the action of IL-6 in clone B ZR-75-1 cells, which are morphologically distinct from wild-type ZR-75-1 cells. In the more polygonal rather than cuboidal clone B cells, IL-6 did not cause early inhibition of DNA synthesis and it caused little cell-cell separation in preformed colonies. However, IL-6 treatment markedly prolonged the interval between mitosis and readherence of daughter cells to their neighbors and the substratum. Supernatants from IL-6-treated cultures contained detached viable cells in increased numbers. Intermitotic intervals were prolonged in IL-6-treated cultures. IL-6-treated dividing breast carcinoma cells are characterized by an increased probability of separation from neighbors and the substratum.
白细胞介素6（IL-6）已被证明可抑制野生型ZR-75-1人乳腺导管癌细胞的增殖，但可增加其运动性，该细胞系与从腹水积液中培养的恶性细胞非常相似。经IL-6处理的细胞失去其上皮特征，形状变为星状或梭形，并从相邻细胞中迁移离开。在野生型ZR-75-1细胞中，IL-6在预先形成的集落中以及有丝分裂后都会导致细胞间分离。我们现在研究了IL-6在克隆B ZR-75-1细胞中的作用，该细胞在形态上与野生型ZR-75-1细胞不同。在更多呈多边形而非立方形的克隆B细胞中，IL-6不会导致DNA合成的早期抑制，并且在预先形成的集落中几乎不会引起细胞间分离。然而，IL-6处理显著延长了有丝分裂与子细胞重新附着于相邻细胞和基质之间的间隔时间。经IL-6处理的培养物的上清液中含有数量增加的脱离的活细胞。在经IL-6处理的培养物中有丝分裂间期延长。经IL-6处理的正在分裂的乳腺癌细胞的特征是与相邻细胞和基质分离的可能性增加。
